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ORIGINAL ARTICLE Table of Contents   
Year : 2008  |  Volume : 51  |  Issue : 4  |  Page : 481-484
Fine-needle aspiration cytology findings in human immunodeficiency virus lymphadenopathy


Department of Pathology, JSS Medical College, Mysore, India

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   Abstract 

Thirty-six human immunodeficiency virus (HIV)-positive patients with lymphadenopathy were subjected to fine-needle aspiration cytology (FNAC) over a period of 2 years. The maximum number of cases was reported in the age group of 21 to 30 years. Majority of the patients were males. The maximum number of cases had tuberculosis (58.3%) followed by reactive lymphadenitis (36.1%), non-Hodgkin's lymphoma (2.7%) and acute suppurative lymphadenitis (2.7%). FNAC is an important diagnostic tool in the evaluation of lymphadenopathy in HIV-positive patients.

Keywords: Human immunodeficiency virus, fine-needle aspiration cytology, granulomatous lymphadenitis, persistent generalized lymphadenopathy

How to cite this article:
Vanisri H R, Nandini N M, Sunila R. Fine-needle aspiration cytology findings in human immunodeficiency virus lymphadenopathy. Indian J Pathol Microbiol 2008;51:481-4

How to cite this URL:
Vanisri H R, Nandini N M, Sunila R. Fine-needle aspiration cytology findings in human immunodeficiency virus lymphadenopathy. Indian J Pathol Microbiol [serial online] 2008 [cited 2020 Apr 1];51:481-4. Available from: http://www.ijpmonline.org/text.asp?2008/51/4/481/43735



   Introduction Top


Acquired immunodeficiency syndrome (AIDS) is known to be caused by a lymphotropic retro-virus, first described by French investigators and later by investigators in United States. AIDS was first recognized in 1981. It has become clear that this syndrome represents the most severe form of a broad spectrum disease. [1] AIDS is a fatal illness that breaks down the body's immunity and leaves the victim vulnerable to life-threatening opportunistic infections, neurological disorders or unusual malignancies. [2] In India, the human immunodeficiency virus (HIV) epidemic is now a decade old and within this short period, it has emerged as one of the most serious public health problems in our country. [3]

Lymphadenopathy is one of the earliest manifestations of HIV. This may be due to the presence and effects of HIV. Lymphadenopathy may also be a manifestation of opportunistic infections, lymphoid malignancy developing in an immunodeficient individual. fine-needle aspiration cytology (FNAC) can serve as an alternative method and may be practiced for the diagnosis of opportunistic infections in HIV/AIDS viz. tuberculosis, histoplasmosis, toxoplasmosis and malignant conditions such as Kaposi sarcoma and lymphoma. [4]

FNAC has become the primary investigative procedure for mass lesions on HIV-positive patients, particularly in the assessment of lymphadenopathy. The procedure is rapid, easily performed and in many cases obviates excision while guiding subsequent therapy or observation. This study was performed to evaluate the role of FNAC as a cytological investigative tool in the diagnosis of various lesions in HIV lymphadenopathy.


   Materials and Methods Top


This study consisted of 36 fine-needle aspiration (FNA) samples obtained from lymph nodes of HIV positive patients confirmed by two enzyme-linked immunosorbent assay (ELISA) tests using different antigens. Aspiration was done as an OPD procedure using a 22-guage needle with standard precautions. Four to five smears were obtained by using multiple passes. Smears obtained were stained with May-Grunwald-Giemsa Stain (MGG), hematoxylin and eosin stain (H and E) and Papanicolaou stain (PAP). Special stains used were Zeil-Neelsen (ZN) stain for acid-fast bacilli (AFB) and periodic acid-Schiff stain (PAS) for fungi.


   Results Top


Thirty-six HIV-positive patients underwent FNAC. After staining with routine cytologic stains and special stains, a detailed cytomorphologic study was conducted.

The various lesions encountered were as follows: Mycobacterium tuberculosis in 20 cases, atypical mycobacterial infection in 1 case, reactive lymphadenitis in 13 cases, non-Hodgkin's lymphoma in 1 case and acute suppurative lymphadenitis in 1 case.

Majority of patients were males in the age group of 21-30 years [Table 1]. Cervical lymph node was the most common site.


   Discussion Top


[Table 2] shows that reactive lymphadenopathy was the most common presentation of HIV cases in western studies. [5],[6] In the present study and as well as in the studies performed by Shenoy et al. [7] and Satyanarayana et al. , [5] mycobacterial infection was more common, possibly because of the increased prevalence of tuberculosis in our country as compared to the developed countries.

The maximum number of cases was found to be in the age group of 21-30 years, followed by 31-40 years. In a study conducted by Bottles et al. , [5] the age of the HIV patients ranged from 18-52 years and the cervical group of lymph nodes were found to be the most commonly affected site. In a study by Bates et al. , [6] 22 males and 1 female were found to be HIV-infected patients and their age ranged from 19 to 72 years. Further, cervical lymph nodes were the most commonly affected site. In a study performed by Shenoy et al. , [7] the male: female ratio was 5:1 and the age group affected was 25-30 years with cervical group of lymph nodes being the most commonly affected site. However, Satyanarayana et al. [8] report axillary node involvement being more common in their study.

In the present study, 21 cases of mycobacterial infection were diagnosed. Ten (47.6%) cases showed epithelioid granulomas and caseation, of which 7 were AFB positive [Figure 1]. Granuloma without caseous necrosis was observed in 6 (28.5%) cases, of which one was AFB positive. Only caseous necrosis was observed in 4 (19.04%) cases, out of which two were AFB positive. In the study conducted by Shenoy et al. , [7] caseous necrosis with epithelioid granuloma was observed in 74% of cases. Granulomas without caseous necrosis was observed in 43% of cases and only caseous necrosis was observed in 8.7% of cases. [7]

One case was diagnosed as Mycobacterium avium-intracellulare (MAI) lymphadenitis that showed aggregates of pale histiocytes with foamy cytoplasm in the smears with poorly formed granulomas. The smear stained positive for both AFB and PAS [Figure 2] and [Figure 3]. Mycobacteria were observed in abundance in the cytoplasm of the histiocytes. The unique feature of Mycobacterium avium-intracellulare is that it stains positively for PAS, as described by Woods and Meyers [9] and was established in our study. In the study performed by Shenoy et al. , [7] one case of MAI lymphadenitis was reported that showed similar findings on smears, as observed in our study.

In the present study, 13 (36.1%) cases of HIV-infected patients presented with reactive lymphadenitis, of which 6 patients presented with persistent generalized lymphadenopathy (PGL). Smears showed polymorphous cell population with mature and transformed lymphocytes, monocytoid cells, neutrophils and tingible body macrophages [Figure 4]. Changes in PGL could not be differentiated from reactive lymph nodes of different etiology in the present study. This finding was also inferred by Bates et al. [6] All the cases of reactive lymphadenitis in the present study were negative for AFB and PAS stains. Satyanarayana et al. [8] report a reactive cytomorphological pattern in 16.4% of their cases of tuberculosis. In the study conducted by Bottles et al. , [5] on HIV lymphadenopathy, 50% of aspirates showed reactive lymphoid hyperplasia. Bates et al. [6] found reactive hyperplasia in 41% aspirates. Ellison et al. [10] and Reid et al. [11] found reactive hyperplasia in 33.3% and 51% aspirates, respectively.

One case (2.7%) of HIV with acute suppurative lymphadenitis was diagnosed. ZN and PAS stains were negative. Smears showed lymphocytes and neutrophils and the aspirate was purulent [Figure 5]. In the study performed by Shenoy et al. , [7] acute suppurative lymphadenitis with AFB positivity was observed in 3 (13%) patients.

The neoplastic lesion reported in the present study was non-Hodgkin's lymphoma found in 1 (2.7%) case. Smear showed sheets of large cells with some of them cleaving [Figure 6]; further typing was not possible. Non-Hodgkin's lymphoma is the most common malignancy found in HIV positive patients 6 . In the study conducted by Saikia et al. , [12] one case of non-Hodgkin's lymphoma was reported. Similarly one case of high-grade B cell lymphoma was reported by Jayaram and Chew. [13]

No cases of Kaposi Sarcoma were found in our study, although it has been reported in the western literature. [5],[6],[11]

In the present study, evidence of opportunistic infections other than Mycobacterium tuberculosis and Mycobacterium-avium-intracellulare was not found in any of the lymph nodes examined. No other opportunistic infections were encountered in the study conducted by Shenoy et al. [7] In the study performed by Bates et al. , [6] one case of Histoplasma and one case of Cryptococcus were found. Satyanarayana et al. [8] reported a case each of Cryptococcus neoformans and Rhodo torula .


   Conclusion Top


FNAC is the primary and safe investigative procedure for lesions of lymph nodes in HIV patients. Procedure is rapid, easily performed and in many cases, it obviates excision, guides subsequent therapy or observation. Most opportunistic infections can be identified and high-grade lymphomas can be diagnosed.

 
   References Top

1.Ewing EP Jr, Chandler FW, Spira TJ, Brynes RK, Chan WC. Primary lymphnode pathology in AIDS and AIDS related lymphadenopathy. Arch Pathol Lab Med 1985;109:977-81.  Back to cited text no. 1  [PUBMED]  
2.Prasad HK, Bhojwani KM, Shenoy V, Prasad SC. HIV manifestations in otolaryngology. Am J Otolaryngol 2006;27:179-85.  Back to cited text no. 2  [PUBMED]  [FULLTEXT]
3.Joshi PL, Rao JV. Changing epidemiology of HIV / AIDS in India. AIDS Res Rev 1999;2:7-9.   Back to cited text no. 3    
4.Shobhana A, Guha SK, Mitra K, Dasgupta A, Negi DK, Hazra SC. People living with HIV infection/AIDS: A study on lymphnode FNAC and CD4 count. Indian J Med Microbiol 2002;2:99-101.  Back to cited text no. 4  [PUBMED]  Medknow Journal
5.Bottles K, McPhaul LW, Volberding P. Fine needle aspiration biopsy of patients with acquired immunodeficiency syndrome (AIDS) experience in an outpatient clinic. Ann Intern Med 1988;108:42-5.  Back to cited text no. 5  [PUBMED]  
6.Martin-Bates E, Tanner A, Suvarna SK, Glazer G, Coleman DV. Use of fine needle aspiration cytology for investigating lymphadenopathy in HIV positive patients. J Clin Pathol 1993;46:564-6.   Back to cited text no. 6  [PUBMED]  [FULLTEXT]
7. Shenoy R, Kapadi SN, Pai KP, Kini H, Mallya S, Khadilkar UN, et al. Fine needle aspiration diagnosis in HIV related lymphadenopathy in Mangalore, India. Acta Cytol 2002;46:35-9.  Back to cited text no. 7  [PUBMED]  
8.Satyanarayana S, Kalghatgi AT, Muralidhar A, Prasad RS, Jawed KZ, Trehan A. Fine needle aspiration cytology of lymph nodes in HIV infected patients. Med J Armed Forces India 2002;58:33-7.   Back to cited text no. 8    
9.Woods Gail L, Meyers Wayne M. Mycobacterial diseases. In: Damjanov I, Linder J, editors. Anderson's pathology. 10 th ed. St. Louri's: Mosby; 1996. p. 843-65.   Back to cited text no. 9    
10.Ellison E, Lapureta P, Martin SE. Fine needle aspiration (FNA) HIV + patients: Results from a series of 655 aspirates. Cytopathology 1998;9:222-9.  Back to cited text no. 10    
11.Reid AJ, Miller RF, Kocjan GL. Diagnostic utility of fine needle aspiration (FNA) Cytology in HIV-infected patients with lymphadenopathy. Cytopathology 1998;9:230-9.   Back to cited text no. 11    
12.Saikia UN, Dey P, Jindal B, Saikia B. Fine needle aspiration cytology in lymphadenopathy of HIV-positive cases. Acta Cytol 2001;45:589-92.  Back to cited text no. 12  [PUBMED]  
13.Jayaram G, Chew MT. Fine needle aspiration cytology of lymphnodes in HIV-infected individuals. Acta Cytol 2000;44:960-6.  Back to cited text no. 13  [PUBMED]  

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Correspondence Address:
H R Vanisri
59/D5, II Main II Cross, Yadavagiri, Mysore - 570 020
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0377-4929.43735

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    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
 
 
    Tables

  [Table 1], [Table 2]

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