LGCmain
Indian Journal of Pathology and Microbiology
Home About us Instructions Submission Subscribe Advertise Contact e-Alerts Ahead Of Print Login 
Users Online: 1922
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
REVIEW ARTICLE
Year : 2009  |  Volume : 52  |  Issue : 2  |  Page : 135-144

Intracytoplasmic antigen study by flow cytometry in hematolymphoid neoplasm


Department of Pathology, Tata Memorial Hospital (TMH), Mumbai, India

Correspondence Address:
Sumeet Gujral
Department of Pathology, Tata Memorial Hospital (TMH), Mumbai
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0377-4929.48902

Rights and Permissions

Flow cytometric detection of intracellular antigens has become a standard method in establishing proper leukemic cell lineage affiliation. It has a non-debatable contribution to the diagnosis of hematolymphoid neoplasm as well as in minimal residual disease. Combination of analysis of fluorescence labeling and light scatter properties of cells allows rapid and better determination of target cell antigens. Regarding the detection of intracellular antigens, standardization of the procedure remains, however, a real challenge. Detection of intracellular antigens by flow cytometry (FCM) requires effective fixation and permeabilization of the cell membrane. In the available literature, some reports describe methodologies to achieve satisfactory results for detection of either cytoplasmic or nuclear antigens; however, no methodological consensus has yet been achieved among the laboratories. This article is an attempt to describe different approaches to detect intracellular molecules by FCM.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed8012    
    Printed279    
    Emailed8    
    PDF Downloaded1117    
    Comments [Add]    
    Cited by others 3    

Recommend this journal