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LETTER TO EDITOR Table of Contents   
Year : 2009  |  Volume : 52  |  Issue : 3  |  Page : 457-458
Seroprevalence of Brucella agglutinins: A pilot study


1 Department of Neuromicrobiology, NIMHANS, Bangalore, India
2 Department of Biotechnology, Manipal Life Sciences Centre, Manipal, India
3 Department of Neuropathology, NIMHANS, Bangalore, India

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Date of Web Publication12-Aug-2009
 

How to cite this article:
Nagarathna S, Sharmada S, Veena Kumari H B, Arvind N, Sundar P, Sangeetha S. Seroprevalence of Brucella agglutinins: A pilot study. Indian J Pathol Microbiol 2009;52:457-8

How to cite this URL:
Nagarathna S, Sharmada S, Veena Kumari H B, Arvind N, Sundar P, Sangeetha S. Seroprevalence of Brucella agglutinins: A pilot study. Indian J Pathol Microbiol [serial online] 2009 [cited 2019 Oct 20];52:457-8. Available from: http://www.ijpmonline.org/text.asp?2009/52/3/457/55036


Sir,

 Brucellosis More Details is a zoonotic disease that continues to be of public health and economic concern in many parts of the world. [1] The reported incidence of human brucellosis worldwide in endemic areas varies widely, from <0.01 to >200 per lakh population .The true incidence however, is unknown and it has been estimated that it may be 25 times higher than the reported incidence due to misdiagnosis and underreporting. Countries like the Mediterranean basin, the Arabian Gulf, the Indian subcontinent and parts of Mexico as well as Central and South America, are especially endemic for human brucellosis. [2]

Human brucellosis is known for protean manifestations, the most common presenting symptom being fever. An unexplained fever is one of the indications of brucellosis and  Brucella More Details agglutination test is one of the screening panels of pyrexia of unknown origin (PUO). Serological investigation of the patients is of paramount importance for diagnosis and future management of the patient. Hence, it is important to know the background prevalence of Brucella antibodies in those who may not be exposed to brucellosis, which might give an overview of the endemicity of the disease. Therefore, the major objective of the present study was to investigate the background prevalence of brucella agglutinins against brucella antigen in blood donor population.

In this prospective study, the samples of blood donors were obtained from Transfusion Medicine Centre, over a period of one month.

A total of 353 samples were analyzed for the presence of brucella antibodies. The age group ranged from 18 to 56 years with 283 males and 70 females giving a male: female ratio of 4:1.

All blood samples were analyzed using Rose Bengal Plate Test (RBPT) and Serum Tube Agglutination Test (STAT). The antigens were procured from Division of Biological Products, Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh. The tests were carried out as per the protocol provided by the manufacturers, which included positive and negative controls. Human brucellosis is a disease endemic in most of the developing countries manifesting as acute, subacute and chronic disease. The transmission of this infection and its predominance in a particular region depends upon several factors like food habits, methods of processing milk and milk products, social customs and husbandry practices, socioeconomic status and environmental hygiene. Due to its varied manifestations, there should be a high index of clinical suspicion for the definitive diagnosis of brucellosis.

The reported incidence and prevalence varies from country to country. A range of prevalence of 1-3.2% from Turkey and 4.5% from Saudi Arabia, has been recorded. [3] Several publications indicate that human brucellosis can be a fairly common disease in India. [4] Varying prevalence of brucella agglutinins, has been reported in different types of population, like 8.5% among dairy personnel, 4.2% in aborted women and 8.5% in human cases in Gujarat. [2]

In our study, out of 353 samples, 52 samples (14.7%) were found to be positive by RBPT method and four samples (1.1%) positive by STAT method, suggesting that RBPT is highly sensitive but less specific. Our results indicate that the specificity of the slide test is enhanced by subjecting the samples to STAT method (4/52=76%). Hence, STAT may remain as the most popular diagnostic tool for brucellosis, worldwide.

There is a paucity of published literature on human brucellosis in India. To the best of our knowledge, there are only few publications projecting the seroprevalence of brucellosis among blood donors and there are none from this part of the country. Vaishnavi et al. , reported a similar study of low seroprevalence of one (0.36%) amongst blood donors at Chandigarh. [5]

In the present study, positivity in four (1.1%) (statistically not significant) samples may be envisaged to be due to consumption of raw milk, travel to regions that are endemic to brucellosis or a brief contact with an infected animal or being from rural background or may be due to cross-reacting antibodies such as Vibrio cholerae,  Yersinia More Details enterocolitica etc.

Our study reports a low seroprevalence of antibodies against brucella agglutinogens, hence may not be suggestive of endemicity of the disease in Bangalore. However, further wide prospective study on a large cluster of samples on long-term basis, needs to be conducted in order to assess the status of the disease in this area.


   Acknowledgment Top


The authors would like to acknowledge Dr. S. K. Shankar, Professor and Head, Department of Neuropathology, NIMHANS for providing the samples, Dr. S. K. Amarnath, Manipal Cure and Care for Statistical Analysis, Dr. M.D Venkatesha, Institute of Animal Health and Veterinary Biologicals, Bangalore, for technical support.

 
   References Top

1.Doganay M, Aygen B. Human Brucellosis: An overview. Int J Infect Dis 2003;7:173-82.  Back to cited text no. 1    
2.Mantur BG, Amarnath SK, Shinde RS. Review of clinical and laboratory features of human Brucellosis. Indian J Med Microbiol 2007;25:188-202.  Back to cited text no. 2  [PUBMED]  Medknow Journal
3.Mantur BG, Biradar MS, Bidri RC, Mulimani MS, Veerappa, Kariholu P , et al. Protean clinical manifestations and diagnostic challenges of human brucellosis in adults: 16 years' experience in an endemic area. J Med Microbiol 2006;55:897-903.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]
4.Mathur TN. Brucella strains isolated from cows, buffaloes, goats, sheep and human beings At Karnal: Their significance with regard to the epidemiology of brucellosis. Indian J Med Res 1964;52:1231-40.  Back to cited text no. 4  [PUBMED]  
5.Vaishnavi C, Kumar S. Investigation for background prevalence of Brucella agglutinins among blood donors. Indian J Med Microbiol 2007;25:302-4.  Back to cited text no. 5  [PUBMED]  Medknow Journal

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Correspondence Address:
S Nagarathna
Department of Neuromicrobiology, NIMHANS, Hosur Road, Bangalore - 29
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0377-4929.55036

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[Pubmed]



 

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