Indian Journal of Pathology and Microbiology
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Year : 2010  |  Volume : 53  |  Issue : 4  |  Page : 846-847
Rushton bodies or hyaline bodies in radicular cysts: A morphologic curiosity


Department of Pathology, Christian Medical College & Hospital, Ludhiana, Punjab, India

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Date of Web Publication27-Oct-2010
 

How to cite this article:
Jacob S. Rushton bodies or hyaline bodies in radicular cysts: A morphologic curiosity. Indian J Pathol Microbiol 2010;53:846-7

How to cite this URL:
Jacob S. Rushton bodies or hyaline bodies in radicular cysts: A morphologic curiosity. Indian J Pathol Microbiol [serial online] 2010 [cited 2014 Sep 16];53:846-7. Available from: http://www.ijpmonline.org/text.asp?2010/53/4/846/72081


Rushton bodies (RB) or hyaline bodies in odontogenic cysts feature as eosinophilic, straight or curved, irregular or rounded structures within the epithelial lining of odontogenic cysts. [1] Data from published studies suggest an overall incidence of about 8%. [2] The origin and nature of RB have been hotly debated.

Two out of 50 cases of odontogenic cysts examined showed the presence of RB. Both were radicular cysts occurring in females aged 40 and 42 years. The anterior maxillary region was involved in both with radiological findings of unilocular radiolucent cysts affecting the apical region of non-vital tooth. The cysts' sizes were 3.5 and 1.5 cm, respectively. They were enucleated.

Histopathologic examination showed the cysts to be lined by stratified squamous epithelium which was variably thickened or attenuated and focally ulcerated. Dense lymphoplasmacytic infiltrate was present in the subepithelial tissues. Tucked within the epithelium were groups of acellular, variable-sized eosinophilic structures (RB) of different shapes from straight linear, curvilinear, to irregular circular forms [Figure 1] and [Figure 2]. Occasional ones had concentric laminations. Some RB were refractile but not birifringent. These bodies were periodic acid-Schiffnegative but strongly positive with Masson's Trichrome and orcein stains [Figure 3]. RB were numerous in one case and few in the other.
Figure 1: (a and b) RB seen as acellular, eosinophilic structures within the squamous lining of radicular cyst. (H and E, ×100)

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Figure 2: RB of varying shapes: circular (a and b); curvilinear (c); irregular (d). (H and E, ×400)

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Figure 3: Hyaline bodies stained positive with orcein (a) and Masson's Trichrome (b) stains

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Hyaline bodies within the epithelium of odontogenic cysts were first described in detail by Rushton [1] and hence are often referred to as Rushton bodies. RB occur almost exclusively within odontogenic cysts. They have not been identified within nasopalatine or fissural cysts, both of which are not of odontogenic origin. [3],[4] There has been only a single case of RB occurring in lesions other than jaw cysts, which was reported within a plexiform ameloblastoma. [5]

RB may show up in examination of the gross specimen as small, white, dome-shaped swellings measuring up to 0.1 mm on the epithelial surface and protruding into the cyst cavity. [6] In microscopic sections, they appear as eosinophilic, linear, straight or curved or hairpin shaped, circular or polycyclic forms, often with a granular core and sometimes concentrically laminated. [2],[6] RB are found almost always within the epithelial lining and only rarely in the fibrous capsule. [6] They stain strongly with orcein, Mallory aldehyde fuschin, Papanicolaou and Gomori. RB within a particular cyst are rarely all of the same shape or type. No association between the cyst type and any specific shape of the RB has been noted. [2] Electron microscopic studies show the lamellar variant to be composed of alternating electron dense and electron lucent layers, whereas the granular form consists of amorphous material. [6],[7]

The origin and nature of RB have been rather elusive, thus leading to numerous studies investigating the histologic, histochemical, enzyme histochemical, transmission and scanning electron microscopic, microprobe, micro-radiographic and immunocytochemical properties. [3],[4] Rushton [1] suggested that RB represented a cuticular or keratin like product of odontogenic epithelium. Alternatively, they have been considered to be of keratinous or of hematogenous origin, which was however disproved by ultrastructural and immunohistochemical studies. [3],[7],[8],[9] Other postulations regarding the origin were that it was formed due to elastotic degeneration or that it was the product of a cellular reaction to extravasated serum. [9] Because of their sole occurrence in odontogenic cysts, the odontogenic epithelium has been strongly implicated in the genesis of RB. It has been concluded after various exhaustive studies that RB are a secretory product of odontogenic epithelium deposited on the surface of particulate matter like cell debris or cholesterol crystals. [2],[3] The focal distribution within the epithelial lining together with the low overall incidence suggests that relatively uncommon local events are responsible. [2]

Thus, the origin of RB seems to have come full circle with the published data available today supporting the view first expressed by Rushton [1] that RB are odontogenic epithelial cell products formed in response to contact with particulate matter in a manner analogous to the formation of dental cuticle.

 
   References Top

1.Rushton MA. Hyaline bodies in the epithelium of dental cysts. Proc R Soc Med 1955;48:407-9.  Back to cited text no. 1
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2.Mathews JB. Hyaline and foreign bodies in cyst walls. In: Browne RM, editor. Investigative pathology of the odontogenic cysts. Boca Raton, Florida CRC Press; 1991. p. 191-210.  Back to cited text no. 2
    
3.Morgan PR, Johnson NW. Histological, histochemical and ultrastructural studies on the nature of hyaline bodies in odontogenic cysts. J Oral Pathol 1974;3:127-47.  Back to cited text no. 3
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4.Yamaguchi A. Hyaline bodies of odontogenic cysts: Histological, biochemical and electron microscopic studies. J Oral Pathol 1980;9:221-34.  Back to cited text no. 4
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5.Takeda Y, Kikuchi H, Suzuki A. Hyaline bodies in ameloblastoma: Histological and ultrastructural observations. J Oral Pathol 1985;14:639-43.  Back to cited text no. 5
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6.Shear M. Radicular cyst, residual cyst, paradental cyst and mandibular infected buccal cyst. In: Shear M, editor. Cysts of the Oral Regions. 3 rd ed. Mumbai: Varghese Publishing House; 1996. p. 136-70.  Back to cited text no. 6
    
7.El Labban NG. Electron microscopic investigation of hyaline bodies in odontogenic cysts. J Oral Pathol 1979;8:81-93.  Back to cited text no. 7
    
8.Shear M. The hyaline and granular bodies in dental cysts. Br Dent J 1961;110:301-7.  Back to cited text no. 8
    
9.Browne RM, Mathews JB. Intra-epithelial hyaline bodies in odontogenic cysts: An immunoperoxidase study. J Oral Pathol 1985;14:422-8.  Back to cited text no. 9
    

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Correspondence Address:
Sunitha Jacob
Department of Pathology, Christian Medical College & Hospital, Ludhiana - 141 008, Punjab
India
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DOI: 10.4103/0377-4929.72081

PMID: 21045441

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