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Year : 2010  |  Volume : 53  |  Issue : 4  |  Page : 897-898
Soil extract media for sporulation of Apophysomyces elegans


Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India

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Date of Web Publication27-Oct-2010
 

How to cite this article:
Mohapatra S, Xess I, Shwetha J V, Choudhary A. Soil extract media for sporulation of Apophysomyces elegans. Indian J Pathol Microbiol 2010;53:897-8

How to cite this URL:
Mohapatra S, Xess I, Shwetha J V, Choudhary A. Soil extract media for sporulation of Apophysomyces elegans. Indian J Pathol Microbiol [serial online] 2010 [cited 2019 Dec 14];53:897-8. Available from: http://www.ijpmonline.org/text.asp?2010/53/4/897/72052


Sir,

Apophysomyces elegans is an emerging zygomycete, reported to cause invasive cutaneous and rhino-orbito-cerebral mucormycosis in immunocompetent individuals. [1] However, it is difficult to identify in routine culture media. Therefore, various special culture media have been suggested. [2],[3] Here, we describe a case of rhino-orbital mucormycosis caused by A. elegans where soil extract media were used for sporulation of the fungus. A 4-year-old boy was admitted to our hospital with complaints of fever, swelling of right eye and severe headache since 7 days. On examination, both upper and lower eyelids were swollen with conjunctival congestion. The vision was intact, and he had no other history of medical illness. Computed tomographic (CT) scan suggested possible fungal invasion in maxillary and ethmoidal sinus without any intracranial extension. Punch biopsy from the site of lesion showed broad and sparsely septate hyphae with 10% KOH mount. White cottony growth was found in culture, which failed to sporulate in subsequent three subcultures on routine media. Spores were not observed in slide cultures with cornmeal agar and potato dextrose agar. Soil extract media [Table 1] at 37°C and 1% agar block media at 25°C were used to enhance sporulation. [4] Growth was observed in both the media after 4 and 12 days of incubation, respectively. It was further confirmed microscopically as A. elegans by lacto-phenol cotton blue (LPCB) mount [Figure 1]. The patient was successfully treated with intravenous amphotericin-B. At 4 months follow-up, he was found to be asymptomatic.
Figure 1: LPCB mount shows broad aseptate hyphae (a) and pyriform-shaped, multi-spored sporangium with flask-shaped apophyses, and hemispherical columella (b)

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Table 1: Composition of soil extract media (pH 6.0-6.2)

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Though most of the Mucorales sporulate on routine culture media such as Sabouroud's dextrose, cornmeal, or potato dextrose agar; A. elegans is known to produce only sterile hyphae. [5] Use of soil extract agar has been proposed to enhance sporulation of A. elegans. [5] However, there is no mention in the literature on the use of this media for this purpose. We compared growth pattern of the fungus using soil extract media in relation to 1% agar block media. The growth of A. elegans was observed much earlier in soil extract media. This medium is easy to prepare, simple to use, and seems to yield early result. It has less chances of contamination in comparison to agar block media. Hence, we suggest routine use of this medium for sporulation of A. elegans. However, further studies are required to prove its utilities in comparison to other sporulating media.

 
   References Top

1.Sridhara SR, Paragache G, Panda NK, Chakrabarti A. Mucormycosis in immunocompetent individuals: An increasing trend. J Otolaryngol 2005;34:402-6.  Back to cited text no. 1
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2.Ellis JJ, Ajello L. An unusual source for Apophysomyces elegans and a method for stimulating sporulation of Saksenaea vasiformis. Mycologia 1982;74:144-5.  Back to cited text no. 2
    
3.Lakshmi V, Rani TS, Sharma S, Mohan VS, Sundaram C, Rao RR, et al. Zygomycotic necrotizing fasciitis caused by Apophysomyces elegans. J Clin Microbiol 1993;31:1368-9.  Back to cited text no. 3
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4.Fisher F, Cook NB, editors. Fundamentals of diagnostic mycology. Philadelphia: W.B. Saunders Co; 1998.  Back to cited text no. 4
    
5.Ribes JA, Vanover-Sams CL, Baker DJ. Zygomycetes in human disease. Clin Microbiol Rev 2000;13:236-301.  Back to cited text no. 5
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Correspondence Address:
Sarita Mohapatra
c/o. Dr. G. P. Rath, F-21, Ansari Nagar (West), AIIMS Residential Campus, New Delhi - 110 029
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0377-4929.72052

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