|Year : 2011 | Volume
| Issue : 1 | Page : 37-41
|Salivary duct carcinoma: Correlation of morphologic features by fine needle aspiration cytology and histopathology
NG Rajesh, Aruna K Prayaga, C Sundaram
Department of Pathology, Nizam's Institute of Medical Sciences, Hyderabad, India
Click here for correspondence address and email
|Date of Web Publication||7-Mar-2011|
| Abstract|| |
Background: Salivary duct carcinoma (SDC) is a highly aggressive primary salivary gland neoplasm that resembles intraductal and infiltrating breast carcinoma. Objectives: To review cytomorphologic features of histology proven SDC and evaluate potential pitfalls in cytologic diagnosis. Materials and Methods: Fine needle aspiration cytology (FNAC) of five histologically proven SDCs were reviewed. Results: One patient was an elderly male (61 years), while the other four patients were younger, in their fourth decade (average age: 38 years). The initial cytologic diagnoses in two of the cases were poorly differentiated carcinoma with differential diagnosis of SDC and high grade mucoepidermoid carcinoma, while in the third case, a possibility of malignant mixed tumor was suggested. In fourth and fifth cases, the diagnosis was suggestive of pleomorphic adenoma with cystic change. The spectrum of cytologic changes included flat sheets and cohesive papillary and three-dimensional clusters. There was moderate to severe nuclear pleomorphism and atypia. Cribriform pattern and necrosis were occasionally identified. Prominent bright granular metachromatic stroma was seen in two of the cases interpreted as pleomorphic adenoma with cystic change and in the tumor reported as suggestive of malignant mixed tumor. The fifth case showed numerous cyst macrophages and apocrine cells with mild nuclear atypia. Conclusion: FNAC of SDC is difficult to interpret because of overlapping cytomorphologic features. Bland cytomorphologic features in some cases and several clinical pitfalls are demonstrated in our series.
Keywords: Fine needle aspiration cytology, mucoepidermoid carcinoma, pleomorphic adenoma, salivary duct carcinoma
|How to cite this article:|
Rajesh N G, Prayaga AK, Sundaram C. Salivary duct carcinoma: Correlation of morphologic features by fine needle aspiration cytology and histopathology. Indian J Pathol Microbiol 2011;54:37-41
|How to cite this URL:|
Rajesh N G, Prayaga AK, Sundaram C. Salivary duct carcinoma: Correlation of morphologic features by fine needle aspiration cytology and histopathology. Indian J Pathol Microbiol [serial online] 2011 [cited 2019 Dec 9];54:37-41. Available from: http://www.ijpmonline.org/text.asp?2011/54/1/37/77321
| Introduction|| |
Salivary duct carcinoma (SDC) is an unusual but very aggressive type of salivary gland carcinoma with a poor prognosis. ,,,,, Early diagnosis and treatment are important as this tumor is known to metastasize early. ,,
SDC is similar histologically to intraductal and infiltrating carcinoma of the breast. Comedonecrosis is a common feature. Aggressive biologic behavior with a high incidence of lymph node metastasis, local recurrence and significant mortality justify categorization of SDC as a high grade malignancy in the current classification of salivary gland neoplasms. 
We analyzed the cytomorphologic features in fine needle aspiration cytology (FNAC) and studied the difficulties related to specific diagnosis and differential diagnosis in five patients with histologically proven SDC.
| Materials and Methods|| |
Five histologically confirmed cases of parotid gland SDCs were identified from histopathology files of our institute. FNAC slides and clinical details of the five patients were retrieved from cytopathology files. FNAC was performed using 23- or 25-gauge needles mounted on 10 ml syringe. All the smears were air dried for May Grunwald Giemsa (MGG) stain and alcohol fixed for Papanicalaou (PAP) stain. All subsequent surgical specimens were fixed in 10% buffered formalin, embedded in paraffin and sectioned at 5 μm and stained with hematoxylin and eosin and alcian PAS stains. FNAC slides were quantified for the following morphologic findings: cellularity, fine cytoplasmic vacuolation, abundant eosinophilic cytoplasm, apocrine cells, indistinct cell borders, nuclear pleomorphism, nuclear membrane irregularity, chromatin clumping, nuclear eccentricity, metachromatic stroma, necrosis, cyst macrophages, mitotic activity, papillary pattern, cribriform configuration and three-dimensional clusters. Among these, cellularity, nuclear pleomorphism, metachromatic stroma and mitotic activity were semiquantitatively assessed using the scores given below.
Semiquantitative Scoring System
The remaining parameters were quantitated for their presence or absence.
- Cellularity: Hypocellular smears +, moderate cellularity ++, high cellularity +++
- Nuclear pleomorphism: Absent -, minimal +, marked pleomorphism ++
- Metachromatic stroma: Absent -, <2 low power field (LPF) +, 2-5 LPF ++, >5 LPF +++
- Mitotic activity/high power field (HPF): Absent -, occasional +, numerous ++
| Results|| |
The clinical findings, initial cytologic and final histopathologic diagnosis are summarized in [Table 1]. Cytologic findings in the five cases with semiquantitative assessment of morphologic findings are presented in [Table 2].
Two tumors (Cases 2 and 3) were interpreted cytologically as poorly differentiated carcinoma with a possibility of SDC. One of the tumors had a differential diagnosis of high grade mucoepidermoid carcinoma. Cells were arranged in sheets and cohesive clusters with abundant eosinophilic cytoplasm, indistinct cytoplasmic boundaries, round to oval pleomorphic nuclei with coarsely clumped chromatin, inconspicuous nucleoli, and nuclear membrane irregularity [Figure 1]a. High mitotic activity, necrosis and dyscohesive tumor cells were also seen. Three-dimensional papillary clusters and cribriform pattern were also seen at foci. The differential diagnosis of mucoepidermoid carcinoma was suggested in view of the younger age of the patient (36 years) coupled with the presence of scattered clusters of tumor cells with vacuolated cytoplasm and rare intracytoplasmic mucin like droplets. Both the cases had bright metachromatic stroma [Figure 1]b prominently seen in MGG stain.
|Figure 1: (a) Sheets of cells with marked nuclear atypia and irregular nuclear contours (PAP, ×200); (b) prominent metachromatic stroma in background (MGG, ×200); (c) cribriform pattern (MGG stain, ×200)|
Click here to view
One tumor (Case 1) was signed out as suggestive of malignant mixed tumor. The patient was a 40-year-old male who had undergone superficial parotidectomy 1 year back at an outside hospital. The histopathology report of the earlier surgical specimen revealed pleomorphic adenoma. The original slides and blocks were not available for review. There was a recurrence of multiple nodules at the surgical site. FNAC showed cellular smears with sheets and three-dimensional clusters of polygonal cells [Figure 1]c. Nuclei were round to oval with coarsely clumped chromatin, irregular nuclear membrane and inconspicuous nucleoli. The cytoplasm showed fine vacuolation in MGG stain [Figure 2]a. The cells were intimately admixed with prominent granular metachromatic stroma.
|Figure 2: (a) vacuolated cytoplasm (MGG, ×400); (b) cells in pseudopapillary clusters, bland nuclear morphology and myxoid matrix (MGG, ×400); (c) numerous cyst macrophages in a background of mucin (MGG, ×200); (d) apocrine cells arranged in cribriform pattern with mild nuclear pleomorphism (PAP, ×200)|
Click here to view
Fourth patient (Case 4) was a 35-year-old female with swelling below the right ear and it was slowly increasing in size. The swelling was nontender with restricted mobility. There was no facial palsy. FNAC smears showed moderate cellularity with clusters of round to oval eccentric nuclei [Figure 2]b admixed with numerous cyst macrophages [Figure 2]c. MGG stained smears showed prominent metachromatic stroma. Nuclear pleomorphism was minimal [Figure 2]d.
The fifth patient (Case 5) was a 42-year-old female with recurrent swelling in the parotid region. The patient had mild pain since few days. There was a history of previous excision 4 years back at an outside hospital. The histopathology slides of the previous excision biopsy were not available for review. FNAC done on the recurrent swelling showed moderately cellular smears with sheets of cyst macrophages and occasional clusters of cells with round to oval nuclei and minimal nuclear pleomorphism. Many cells also showed apocrine change [Figure 2]d. The background showed bright metachromatic stroma in MGG stain.
Histologically, the first four tumors had features of high grade SDC with prominent comedonecrosis and high mitotic activity. Two of the tumors revealed lymphatic and perineural invasion.
Fifth case was low grade SDC and it was cystic on gross examination and showed intraductal proliferation exhibiting cystic ducts with micropapillary, tufted and plaque like intraluminal proliferation. Some of the ducts were distended by solid or pseudocribriform proliferation with varied cystic dilatation and exhibited mild nuclear atypia. There were no goblet cells and mucin stains were negative.
| Discussion|| |
SDC is an uncommon but distinctive type of salivary gland tumor that mimics the histologic patterns of intraductal and infiltrating ductal carcinoma of the breast. It is one of the most aggressive types of salivary gland carcinoma with a marked tendency toward distant metastases and local recurrence. Kleinsasser et al. first described SDC in 1968. Patients commonly present with rapidly growing mass, often with facial nerve involvement, localized pain and cervical adenopathy. , Approximately, 76% of the patients reported with this condition have been men. The age range at presentation is 22-91 years with a peak incidence in the sixth and seventh decades.  Young age at diagnosis, primary tumor >3.0 cm and origin in submandibular gland, all worsen the prognosis. , Even with aggressive therapy, prognosis is poor with a mortality rate of 60-70%. ,,,,
On gross examination, the tumors are usually poorly circumscribed and often multinodular, sometimes with scattered cysts and focal areas of necrosis. They often induce fibrotic reaction. 
Mucicarmine and alcian blue PAS stains occasionally demonstrate luminal or interstitial reactivity, but the tumor cells generally are negative although Hui et al.  reported occasional intracellular mucin. Perineural infiltration and lymphatic infiltration are frequently present.
Twenty five cases describing the cytomorphology of SDC by FNAC have appeared in the literature. ,,,,, The cytomorphologic features described in this reports include broad flat sheets of cells, tightly cohesive three-dimensional clusters, papillary and cribriform configurations, large polygonal or low columnar cells with abundant, granular cytoplasm and eccentric, hyperchromatic nuclei. Conversely, polygonal cells with round to oval hypochromatic nuclei having prominent nucleoli have also been described. Nuclear atypia has ranged from mild to moderate to "malignant". A necrotic background has been observed in some, but not all cases. A report by Elsheikh et al. discusses the differential diagnostic considerations in SDC by FNAC. These authors included high grade mucoepidermoid carcinoma, acinic cell carcinoma, oncocytic neoplasms, papillary cystadenocarcinoma and polymorphous low grade adenocarcinoma in the differential diagnosis.
Although oncocytic neoplasms are the only benign entities in this differential diagnosis, Elsheikh et al. mention only briefly the characteristic features distinguishing SDC from oncocytic neoplasms. They stated that SDC usually shows a higher nuclear/cytoplasmic ratio, less granular cytoplasm and many three-dimensional clusters. They emphasized that cribriform pattern and comedonecrosis are absent from oncocytic lesions. 
A series by Fyrat et al. included one case that was misdiagnosed as an oncocytic neoplasm and another case in which atypical Warthin tumor was included in the differential diagnosis. However, it can be difficult to distinguish apocrine cells from oncocytes in cytologic smears and oncocytes are much more commonly observed in salivary gland aspirates. Both apocrine cells and oncocytes exhibit granular cytoplasm and round nuclei with prominent nucleoli, but oncocytes generally show more finely granular cytoplasm than apocrine cells. The later may exhibit apical cytoplasmic protrusions or snouts which are not seen in oncocytes, but such apical protrusions may be more evident in histologic or cell block material than in smears. 
The authors concluded in retrospect that the presence of cribriform areas and a background of necrosis should have prompted a consideration of SDC in the first case. Oncocytic appearing areas in SDC are generally apocrine rather than being truly oncocytic. 
The most useful feature for distinction SDC from an oncocytic neoplasm by FNAC is the presence of cribriform groups. Absence of cribriform and papillary groups, however, leads to inconclusive diagnosis and, as noted in previous reports, this feature is also more apparent on cell block material than in histologic smears. ,,,
High grade mucoepidermoid carcinoma was described by Elsheikh et al. as the most difficult neoplasm to be distinguished cytologically from SDC. In the absence of papillary groups or cribriform areas, one may not be able to distinguish such an aspirate from high grade mucoepidermoid carcinoma or squamous carcinoma. 
None of the three subsequent reports describing the cytomorphologic findings on SDC confirmed the presence of intranuclear inclusions or emphasized the importance of nuclear eccentricity. ,,
Some of the previous reports of SDC claim that cytologic findings are sufficiently characteristic to suggest a specific diagnosis.
In general, FNAC of parotid masses have a high accuracy with over 99% specificity and 85% sensitivity. , Immunohistochemistry (IHC) with AR, GCDFP-15 and p63, as well as morphometric analysis of these tumors can be very useful adjuncts in cytologic diagnosis of SDCs. Mucin rich variant of SDC with large pools of extracellular mucin has been described and this variant carries poor prognosis. IHC with carcinoembryonic antigen (CEA), cytokeratins, S-100 protein, estrogen receptor (ER), progesterone receptor (PR) and HER 2/neu have low sensitivity and specificity, while mucin antigen profile MUC2, MUC5B, and MUC6 have shown positivity in mucin rich variant. IHC studies have revealed a consistent expression of androgen receptor (AR) and GCDFP-15. Negative expression of p63 is another sensitive test in the diagnosis of SDC. ,
Gal et al. described intranuclear inclusions and nuclear eccentricity as a characteristic cytologic finding in SDC. He also described naked nuclei with anisokaryosis, chromatin clumps and clear vacuolar zones in his case report.
None of our five cases demonstrated the presence of intranuclear inclusions though nuclear eccentricity was observed in one of the five cases.
Klijanienko  described intranuclear vacuoles and binucleate cells. He also described abundant cytoplasm with basophilic cytoplasm on MGG stain with numerous cytoplasmic vacuoles in addition to oncocytic cells with abundant eosinophilic cytoplasm. Some of the tumors showed dense mucoid background with microvacuolated cells and extensive necrosis, resulting in misdiagnosis with mucoepidermoid carcinoma and squamous cell carcinoma, respectively. 
Morphometric analysis of area of nucleus revealed statistically significant differences with nuclear area in SDC being larger than high and intermediate grade mucoepidermoid carcinomas while being smaller than that of squamous cell carcinoma ex-pleomorphic adenoma.  SDC is more common in men with a peak incidence in sixth and seventh decades. 
In our series, we observed that four of the five cases were patients presenting at a younger age group and two were females. The relative morphologic and clinical heterogeneity of SDC may preclude specific cytologic diagnosis. Although adequate sampling may ensure diagnosis of a high grade carcinoma with a possibility of SDC, the distinction of SDC with mild nuclear atypia or low grade histology may be difficult or impossible on the basis of cytomorphology alone.  However, ancillary studies such as quantitative morphometry, IHC with a panel of antibodies, AR, GCDFP-15 and p63, are helpful in the definitive diagnosis of SDC by FNAC.
| References|| |
|1.||Afzelius LE, Cameron WR, Svenson C. Salivary duct carcinoma: A clinicopathologic study of 12 cases. Head Neck 1987;9:151-6. |
|2.||Anderson C, Muller R, Piorkowski R, Knibbs DR, Vignoti P. Intraductal carcinoma of major salivary gland. Cancer 1992;69:609-14. |
|3.||Brandwein MS, Jagirder J, Patil J, Biller H, Kaneko M. Salivary duct carcinoma (cribriform salivary carcinoma of excretory ducts): A clinicopathologic and immunohistochemical study of 12 cases. Cancer 1990;65:2307-14. |
|4.||Butterworth DM, Jones AW, Kotecha B. Salivary duct carcinoma: Report of a case and review of the literature. Virchows Arch 1992;420:371-4. |
|5.||Chen KT. Intraductal carcinoma of the minor salivary gland. J Laryngol Otol 1983;97:189-91. |
|6.||Delgado R, Vuitch F, Albores-Saavedra J. Salivary duct carcinoma. Cancer 1993;72:1503-12. |
|7.||Seifert G, Batsakis JG, Brocheriou C, Cardesa A, Dardict I, Ellis GL, et al. World Health Organization. Histological typing of salivary gland tumors. 2nd ed. Berlin: Springer-Verlag; 1991. |
|8.||Kleinsasser O, Klein HJ, Hubner G. Salivary duct carcinoma: A group of salivary gland tumors analogous to mammary duct carcinoma. Arch Klin Exp Ohren NasenKehlkofheild 1968;192:100-5. |
|9.||Lewis JE, Mckinney BC, Weiland LH, Ferreiro JA, Olsen KD. Salivary duct carcinoma: Clinicopathologic and immunohistochemical review of 26 cases. Cancer1996;77:223-30. |
|10.||Ellis GL, Auclair PL. Malignant epithelial tumors. Atlas of tumor pathology: Tumors of salivary glands. Washington, DC: Armed Forces Institute of Pathology; 1996. p. 324-33. |
|11.||Garland TA, Innes DJ, Fechner RE. Salivary duct carcinoma: An analysis of four caseswith review of literature. Am J Clin Pathol 1984;81:436-41. |
|12.||Hellquist HB, Karlsson MG, Nilsson C. Salivary duct carcinoma: A highly aggressivesalivary duct tumor with overexpression of C-erb-2 protein. J Pathol 1994;172:35-44. |
|13.||Zohar Y, Shem-Tov Y, Gal R. Salivary duct carcinoma in major and minor salivary glands, a clinicopathologic analysis of four cases. J Craniomaxillofac Surg 1988;6:320-3. |
|14.||Ellis GL, Auclair PL, Gnepp DR, Goode RK. Other malignant epithelial neoplasms. In: Ellis GL, Auclair PL, Gnepp DR, editors. Surgical pathology of the salivary glands. Major problems in pathology. Vol. 25. Philadelphia: W.B. Saunders; 1991. p. 476-80. |
|15.||Ellis GL, Auclair PL. Tumors of the Salivary Glands. Atlas of Tumor Pathology. 3rd Series, Fascicle 17. Washington, DC: Armed Forces Institute of Pathology; 1996. p. 325. |
|16.||Hui KK, Batsakis JG, Luna MM, Mackay B, Byers RM. Salivary duct carcinoma (cribriform salivary carcinoma of excretory ducts). A high grade malignancy. J Laryngol Otol 1986;100:105-14. |
|17.||Colecchia M, Frigo B, Leopardi OM. Salivary duct carcinoma of the parotid gland: Report of a case with cytologic and immunocytochemical findings on fine needle aspirationbiopsy. Acta Cytol 1997;41:593-7. |
|18.||Dee S, Masood S, Issacs JH Jr, Hardy NM. Cytomorphologic features of salivary ductcarcinoma on fine needle aspiration biopsy: A case report. Acta Cytol 1993;37:539-42. |
|19.||Elsheikh TM, Bernacki EG Jr, Pisharodi L. Fine needle aspiration cytology of salivary ductcarcinoma. Diagn Cytopathol 1994;11:47-51. |
|20.||Fyrat P, Cramer H, Feczko JD, Kratzer S, Layfield LJ, Eisenhut CC, et al. Fine needle aspiration biopsy of salivary duct carcinoma. Report of five cases. Diagn Cytopathol 1997;16:526-30. |
|21.||Garcia-Bonafe M, Catala I, Tarragana J, Tallada N. Cytologic diagnosis of salivary duct carcinoma: A review of seven cases. Diagn Cytopathol 1998;19:120-3. |
|22.||Khurana KK, Pitman MB, Powers CN, Korourian S, Bardales RH, Stanley MW. Diagnostic pitfalls of aspiration cytology of salivary duct carcinoma. Cancer Cytopathol1997;81:373-8. |
|23.||Ersoz C, Cetik F, Aydin O, Cosar EF, Talas DU. Salivary duct carcinoma ex pleomorphic adenoma: Analysis of the findings in fine-needle aspiration cytology and histology. Diagn Cytopathol 1998;19:201-4. |
|24.||Zurrida S, Alasio L, Tradati N, Bartoli C, Chiesa F, Pilotti S. Fine-needle aspiration of parotid masses. Cancer 1993;72:2306-11. |
|25.||Orell SR. Diagnostic difficulties in the interpretation of fine needle aspirates of salivary gland lesions: The problem revisited. Cytopathology 1995;6:285-300. |
|26.||Simpson RH, Prasad AR, Lewis JE, Skálová A, David L. Mucin rich variant of salivary duct carcinoma: A clinicopathologic and immunohistochemical study of four cases. Am J Surg Pathol 2004;27:1070-9. |
|27.||Moriki T, Ueta S, Takahashi T, Mitani M, Ichien M. Salivary duct carcinoma: Cytologic characteristics and application of androgen receptor immunostaining for diagnosis. Cancer 2001;93:344-50. |
|28.||Gal R, Strauss M, Zohar Y, Kessler E. Salivary duct carcinoma of the parotid gland.Cytologic and histopathologic study. Acta Cytol 1985;29:454-6. |
|29.||Klijanienko J, Vielh P. Cytologic characteristics and histomorphologic correlations of 21 salivary duct carcinomas. Diagn Cytopathol 1998;19:333-7. |
|30.||Kawahara A, Harada H, Akiba J, Kage M. Salivary duct carcinoma cytologically diagnosed distinctly from salivary gland carcinomas with squamous differentiation. Diagn Cytopathol 2008;36:485-93. |
|31.||Gilcrease MZ, Guzman-Paz M, Froberg K, Pambuccian S. Salivary duct carcinoma. Is a specific diagnosis possible by fine needle aspiration cytology? Acta Cytol 1998;42:1389-96. |
|32.||Delgado R, Klimstra D, Albores-Saavedra J. Low grade salivary duct carcinoma: A distinctive variant with a low grade histology and a predominant intraductal growth pattern.Cancer 1996;78:958-67. |
Aruna K Prayaga
Department of Pathology, Nizam's Institute of Medical Sciences, Hyderabad - 500 082
Source of Support: None, Conflict of Interest: None
[Figure 1], [Figure 2]
[Table 1], [Table 2]
|This article has been cited by|
||Fine-needle aspiration cytology of the apocrine variant of epithelial-myoepithelial carcinoma
| ||Diandra Perez,Rana Naous |
| ||Diagnostic Cytopathology. 2019; |
|[Pubmed] | [DOI]|
||Nodular oncocytic hyperplasia: Can cytomorphology allow for the preoperative diagnosis of a nonneoplastic salivary disease?
| ||Lisa M. Rooper,Mine Onenerk,Momin T. Siddiqui,William C. Faquin,Justin A. Bishop,Syed Z. Ali |
| ||Cancer Cytopathology. 2017; 125(8): 627 |
|[Pubmed] | [DOI]|
||The Value of Ultrasound-Guided Fine-Needle Aspiration Cytology by Cytopathologists in the Diagnosis of Major Salivary Gland Tumors
| ||Shahrzad Negahban,Sadegh Shirian,Bijan Khademi,Ahmad Oryan,Roshanak Sadoughifar,Mohammadian-Panah Mohammad,Azita Aledavood,Khosrow Daneshbod,Yayha Daneshbod |
| ||Journal of Diagnostic Medical Sonography. 2016; 32(2): 92 |
|[Pubmed] | [DOI]|
| Article Access Statistics|
| Viewed||5688 |
| Printed||156 |
| Emailed||3 |
| PDF Downloaded||442 |
| Comments ||[Add] |
| Cited by others ||3 |