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ORIGINAL ARTICLE
Year : 2011  |  Volume : 54  |  Issue : 2  |  Page : 330-334

Clonality assessment of lymphoproliferative lesions using the polymerase chain reaction: An analysis of two methods


Department of Pathology, Armed Forces Medical College, Pune, India

Correspondence Address:
Nikhil Moorchung
Department of Pathology, Armed Forces Medical College, Sholapur Road, Pune - 411 040
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0377-4929.81628

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Background: Lymphoid malignancies are a heterogeneous group of disorders which may be difficult to differentiate from reactive proliferations even after immunohistochemistry. Polymerase chain reaction (PCR) is believed to be a good adjunct tool for diagnosis. Materials and Methods: We examined 24 cases of neoplastic and non-neoplastic lymphoproliferative lesions in this study and evaluated the PCR as an additional tool in the confirmation of the diagnosis. Two different PCR methodologies were evaluated. Results: In the evaluation of the T-cell PCR, it was seen that the correlation using both the commercial kits and the custom-synthesized primers was highly significant at a P value of <0.05. In the evaluation of the B-cell PCR, it was seen that the correlation using both the commercial kits and the custom-synthesized primers was not significant using either method (P > 0.05). Conclusions: Both the methods showed an excellent concordance for T-cell γ gene rearrangements, However, the same was not seen in the B-cell receptor rearrangements. This may be because of the small sample size or the inability of consensus V primers to recognize complementary DNA sequences in all of the V segments.


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