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Indian Journal of Pathology and Microbiology
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ORIGINAL ARTICLE
Year : 2012  |  Volume : 55  |  Issue : 2  |  Page : 175-179

HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization


Division of Molecular Pathology, Department of Pathology, Tata Memorial Hospital and Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Mumbai, India

Correspondence Address:
Sridhar Epari
Department of Pathology, Tata Memorial Hospital and Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Mumbai, Maharashtra
India
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Source of Support: The project was funded by our institutional intramural grant (project 441)., Conflict of Interest: None


DOI: 10.4103/0377-4929.97855

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Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH). Recent studies have shown chromogenic in-situ hybridization (CISH) as a relatively cheaper alternative. Materials and Methods : Forty-three nonconsecutive, randomly selected primary invasive breast cancer cases were evaluated for c-erbB-2 (HER2 protein) by IHC and gene amplification by FISH and CISH. Results of each of the same were compared. Results : CISH showed approximately 90% and 100% concordance for IHC negative and positive cases, respectively; while approximately 94.4% and 91% concordance with FISH amplified and non-amplified cases, respectively. Conclusion : This study showed feasibility of incorporation of CISH as a low cost option in routine management of breast carcinoma in the Indian setting. Secondly, reconfirmation of IHC negative and positive cases can be done by CISH.


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