Indian Journal of Pathology and Microbiology
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Year : 2013  |  Volume : 56  |  Issue : 3  |  Page : 243-247

Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis

School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, P.R. China

Correspondence Address:
Li Wang
Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province - 130 024, P.R. China

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Source of Support: This work was supported by grants from the National Natural Science Foundation of China (30872365, 81028010), Jilin provincial Government of the People’s Republic of China (20100949) and the Bureau of Science and Technology of Changchun, China (2010058), Conflict of Interest: None

DOI: 10.4103/0377-4929.120377

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Background: The secreted aspartyl proteinases 2 (Sap2) of Candida albicans (C. albicans) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion. Aim: In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA). Materials and Methods: Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL. Results: Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was simple and economical to perform and had a level of sensitivity for detection of low-titer positive sera; thus, it may be proven to be of value in epidemiological studies on candidiasis.

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