Indian Journal of Pathology and Microbiology
Home About us Instructions Submission Subscribe Advertise Contact e-Alerts Ahead Of Print Login 
Users Online: 2296
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
Year : 2013  |  Volume : 56  |  Issue : 3  |  Page : 269-271

Development and evaluation of flavi-immunoglobulinM capture enzyme-linked immunosorbent assay

Department of Virology, King Institute of Preventive Medicine and Research, Guindy, Chennai, Tamil Nadu, India

Correspondence Address:
Kaveri Krishnasamy
Department of Virology, King Institute of Preventive Medicine and Research, Guindy, Chennai - 600 032, Tamil Nadu
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0377-4929.120391

Rights and Permissions

In this study, we report the evaluation of In-house flavi virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA), which can be used as a screening test to determine the infecting flavivirus serotype over the current serological methods. A panel of 88 sera (inclusive of well characterized dengue, Japanese Encephalitis (JE) and West Nile virus (WNV) positive and negative samples tested and confirmed by commercial kit) was used for evaluation of the kit. The sensitivity and specificity of the In-house capture assay versus the commercial kit for the sero-diagnosis of dengue was 100% and 87% respectively, for JE IgM, it was found to be 90% and 100% respectively, and for West Nile it was 87.5% and 90.9%. Based on the study, we concluded that this flavivirus-serotyping ELISA provides rapid results and may be used as an accurate alternate to other serological tests for the specific diagnosis of flavivirus infections.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded153    
    Comments [Add]    
    Cited by others 1    

Recommend this journal