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  Table of Contents    
LETTER TO EDITOR  
Year : 2014  |  Volume : 57  |  Issue : 1  |  Page : 158
Sensitivity testing of Staphylococcus aureus


Department of Microbiology, Indira Gandhi Government Medical College, Central Avenue, Nagpur, Maharashtra, India

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Date of Web Publication17-Apr-2014
 

How to cite this article:
Agrawal GN, Wandre AS. Sensitivity testing of Staphylococcus aureus. Indian J Pathol Microbiol 2014;57:158

How to cite this URL:
Agrawal GN, Wandre AS. Sensitivity testing of Staphylococcus aureus. Indian J Pathol Microbiol [serial online] 2014 [cited 2019 Dec 12];57:158. Available from: http://www.ijpmonline.org/text.asp?2014/57/1/158/130938


Sir,

We want to congratulate Thool et al. for sharing the data on Staphylococcus aureus infecting orthopedic patients. [1] Although the work is carefully and critically done some clarifications are needed.

Having carefully gone through the article, it was observed that methicillin sensitivity for S. aureus isolates was performed using the methicillin disc and reference of Clinical and Laboratory Standards Institute (CLSI) 2006 was quoted. [2] CLSI 2006 and CLSI 2011 state that methicillin is no longer the agent of choice for testing methicillin resistance in S. aureus isolates and advocate the use of cefoxitin to report methicillin resistance. [2],[3]

In the study, [1] as many as 11 strains of S. aureus were reported as vancomycin resistant depending on the results of disc diffusion test only. CLSI 2006 states that staphylococcal isolates for which vancomycin zone diameters are 14 mm or less (resistant) should be tested by a reference minimum inhibitory concentration (MIC) method. [2] CLSI 2011 proclaims that the disc test is not reliable for testing vancomycin in S. aureus and MIC test should be performed to determine the susceptibility of all isolates of staphylococci to vancomycin. [3]

In the study, [1] staphylococcal isolates were considered linezolid resistant, if the zone of inhibition was 21 mm or less. CLSI 2006 and CLSI 2011 mention that the isolates should be considered sensitive if the zone of inhibition is 21 mm. [2],[3]

Through this letter, we would like to emphasize strict adherence to CLSI guidelines that serve as standard for uniformity and are based on the sound technical background. This will also avoid misleading the clinicians for the choice of drug for effective patient management.

 
   References Top

1.Thool VU, Bhoosreddy GL, Wadher BJ. Detection of resistance to linezolid in Staphylococcus aureus infecting orthopedic patients. Indian J Pathol Microbiol 2012;55:361-4.  Back to cited text no. 1
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2.Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing 16 th Informational supplement. Vol. 25, No. 1. Wayne PA: Clinical and Laboratory Standards Institute 2006. M100-S16.  Back to cited text no. 2
    
3.Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing 21 st Informational Supplement. Vol. 31, No. 1. Wayne PA: Clinical and Laboratory Standards Institute; 2011. M100-S21  Back to cited text no. 3
    

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Correspondence Address:
Gopal N Agrawal
75 Hanuman Nagar, Nagpur - 440 009, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0377-4929.130938

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