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ORIGINAL ARTICLE
Year : 2015  |  Volume : 58  |  Issue : 1  |  Page : 36-39

Comparison of four diagnostic techniques for detection of Trichomonas vaginalis infection in females attending tertiary care hospital of North India


1 Department of Microbiology, Era's Lucknow Medical College and Hospital, Lucknow; Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, A.M.U., Aligarh, Uttar Pradesh, India
2 Department of Community Medicine, Teerthanker Mahaveer Medical College and Research Center, Teerthanker Mahaveer University, Moradabad, Uttar Pradesh, India
3 Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, A.M.U., Aligarh, Uttar Pradesh, India
4 Department of Obstetrics and Gynecology, Jawaharlal Nehru Medical College and Hospital, A.M.U., Aligarh, Uttar Pradesh, India

Correspondence Address:
Dr. Noor Jahan
Department of Microbiology, Era's Lucknow Medical College and Hospital, Lucknow - 226 003
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0377-4929.151172

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Background: Trichomonas vaginalis causes a common sexually transmitted disease trichomoniasis, which may lead to increased risk of transmission of human immunodeficiency virus infection and other pelvic inflammatory diseases. Wet mount examination is the most common test for diagnosis, but it has low sensitivity. Acridine orange staining can be used for diagnosis, but it requires special microscopic facility. Culture is considered as the gold standard, but it takes a long time for diagnosis. OSOM Trichomonas Rapid Test is a recently introduced rapid method based on immunochromatographic assay of trichomonal protein antigens. Hence, the present study was done to compare these four diagnostic techniques for detection of trichomoniasis in females with vaginal discharge. Materials and Methods: Vaginal swabs were taken from 835 female patients and wet mount examination, acridine orange staining, culture in Kupferberg medium, and OSOM Trichomonas Rapid Test, were performed. Results: Out of 835 patients included in our study, 68 (8.1%) positive cases of trichomoniasis were detected by culture. OSOM Trichomonas Rapid Test detected 63 (7.5%) cases, acridine orange staining detected 53 (6.3%) cases, whereas, wet mount examination detected only 45 (5.4%) positive cases. OSOM Trichomonas Rapid Test performed well and showed high sensitivity and specificity of 88.2% and 99.6%, respectively. Conclusion: As OSOM Trichomonas Rapid Test is a point of care test and gave better results than both wet mount examination and acridine orange staining; it can be used as a routine test in peripheral areas lacking laboratory facilities.


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