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Indian Journal of Pathology and Microbiology
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ORIGINAL ARTICLE
Year : 2015  |  Volume : 58  |  Issue : 1  |  Page : 40-44

Comparison of immunofluorescence and enzyme-linked immunosorbent assay and immunoglobulin G avidity techniques for screening of anti: Toxoplasma antibodies among single serum sample pregnant women in Tabriz, Iran


1 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5 Department of Parasitology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Correspondence Address:
Shohreh Almasi
Immunology Research Center, Tabriz University of Medical Sciences, Golgasht St., Daneshgah St., Tabriz
Iran
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Source of Support: This study was financially supported by Tropical and Infectious Diseases Research Center (TIDRC), Tabriz, Iran. The authors would like to thank the staff and authorities in TIDRC for supporting the conduct of this research. We are grateful to research development and coordination center (RDCC) in Tabriz University of medical sciences., Conflict of Interest: None


DOI: 10.4103/0377-4929.151183

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Background: Congenital toxoplasmosis is that pregnant women acquire the infection during gestation; diagnosis of the acute infection during pregnancy is a complex subject of maternal toxoplasmosis. Thus, the presence of immunoglobulin G (IgG) and/or IgM Toxoplasma antibodies in a single serum sample drawn during gestation cannot be used to define whether the infection was recently acquired or chronic. Materials and Methods: At this cross-sectional descriptive study, sera of 391 pregnant women examined and compared. They were in an age range of 21-35 years, referred by gynecologists and infectious disease specialists, during March 2012-April 2013. They have referred, 215 (54.98%), 102 (26%), 74 (18.92%) in the first, second and third trimesters of gestation, respectively. For each of them, a questionnaire was completed and serum samples were prepared in an equal condition, examined according to the procedures of indirect immunofluorescence (IIF), enzyme-linked immunosorbent assay (ELISA) and IgG Avidity techniques. Results: We have found 111 (28.38%) seronegative and 280 (71.61%) seropositive cases by IIF and 124 (31.70%) seronegative, 267 (68.28%) seropositive cases by ELISA. The IgG avidity test confirmed 45 (69.23%) and 7 (10.76%) doubtful cases of IgM test in IIF and ELISA techniques. Conclusions: This study highlights how to manage pregnant women with toxoplasmosis, especially in a single serum sample condition.


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