LGCmain
Indian Journal of Pathology and Microbiology
Home About us Instructions Submission Subscribe Advertise Contact e-Alerts Ahead Of Print Login 
Users Online: 2352
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
IJPM is coming out with a Special issue on "Genitourinary & Gynecological pathology including Breast". Please submit your articles for these issues


 
  Table of Contents    
CASE REPORT  
Year : 2017  |  Volume : 60  |  Issue : 4  |  Page : 599-600
A rare case of Shewanella putrefaciens bacteremia in a patient of road traffic accident


1 Department of Surgery, Acharya Shree Bhikshu Government Hospital, New Delhi, India
2 Department of Microbiology, ESI-PGIMSR, Basaidarapur, New Delhi, India

Click here for correspondence address and email

Date of Web Publication12-Jan-2018
 

   Abstract 


Shewanella putrefaciens rarely causes human infection. These are mostly found in environment and food stuffs. Shewanella are often found in mixed culture. It has been implicated in cellulitis, otitis media, and septicemia. It may be found in respiratory tract, urine, feces, and pleural fluid. There is no definite guideline for therapeutic option. In general, these are susceptible to various antimicrobial agents but are often resistant to penicillin and cephalothin. We report a rare case of bacteremia by S. putrefaciens in a patient of head injury with polytrauma after a road traffic accident.

Keywords: Road traffic accident, Shewanella algae, Shewanella putrefaciens

How to cite this article:
Ranjan R, Chowdhary P. A rare case of Shewanella putrefaciens bacteremia in a patient of road traffic accident. Indian J Pathol Microbiol 2017;60:599-600

How to cite this URL:
Ranjan R, Chowdhary P. A rare case of Shewanella putrefaciens bacteremia in a patient of road traffic accident. Indian J Pathol Microbiol [serial online] 2017 [cited 2019 Sep 20];60:599-600. Available from: http://www.ijpmonline.org/text.asp?2017/60/4/599/222965





   Introduction Top


Shewanella putrefaciens is a Gram-negative bacterium. It has been isolated from marine environments as well as anaerobic sandstone in the Morrison formation in New Mexico.[1]

Although it is very rare for it to act as a human pathogen, there have been cases of infections and bacteremia caused by S. putrefaciens.[2] Much of the problem in prevention comes from tendency of Shewanella to become a contaminant or saprophyte, meaning, it is often living among other bacterial infections on previously damaged organs, as well as the bacteria's ability to survive at extreme low temperatures and respiratory diversity.[3] Very few case reports of S. putrefaciens bacteremia have been reported from India. We report a rare case of bacteremia by S. putrefaciens in a patient of head injury with polytrauma after a road traffic accident.


   Case Report Top


A 24-year-old male patient with head injury with polytrauma was admitted in our hospital following road traffic accident. His Glasgow Coma Scale was poor at the time of admission with computerized tomographic scan of brain showing multiple contusions. He had bilateral clavicle fracture, left ulna fracture, and radius fracture. Maxillofacial bones were also fractured. The patient was intubated immediately and put on ventilator support and treatment started. Intravenous piperacillin/tazobactam and intravenous gentamycin were started for broad-spectrum bacterial coverage. Intravenous phenytoin and fluids were also started. His blood, urine, and endotracheal aspirate sample came to our laboratory for culture and sensitivity. Urine and endotracheal aspirate were sterile on culture. Blood culture by BacT/ALERT 3D ® system (bioMerieux Inc., 100 Rodolphe Street, Durham, NC, USA) was done. Hematological investigations revealed white cell count of 14.5 × 109/L and hemoglobin level of 13.0 g/dL. Blood urea was 39 mg/dL, and blood sugar was 77 mg/dL. Serum electrolytes were sodium 140 mEq/L, potassium 4.4 mmol/L, and calcium 7.3 mg/dL.

Blood culture showed convex, circular, brown tan colored colonies on blood agar plate [Figure 1]. On MacConkey agar plate, pale colonies were obtained. Gram stain showed Gram-negative bacilli. The organism was positive for motility test, oxidase reaction, and produced hydrogen sulfide in butt of Triple sugar agar. Nitrate was reduced to nitrite, and ornithine was decarboxylated. The isolate was identified as Shewanella by standard bacteriological techniques. Identification was confirmed by VITEK ® 2 system (bioMerieux Inc., 100 Rodolphe Street, Durham, NC, USA) as S. putrefaciens. Antibiotic susceptibility was done by Kirby–Bauer method. The isolate was susceptible to all common antibiotics used for Gram-negative coverage used according to Clinical and Laboratory Standards Institute guidelines.
Figure 1: Growth on blood agar

Click here to view


The patient was not saved despite our best efforts. He had polytrauma along with head injury. He was not weaned from ventilator and was declared dead 15 days after admission.


   Discussion Top


S. putrefaciens is a Gram-negative, nonfermentative, oxidase-positive, motile bacillus that produces hydrogen sulfide. It is found widely in the nature especially in marine environments. In some very rare cases, S. putrefaciens can be a human pathogen. It can produce a wide variety of clinical syndromes including bacteremia as well as skin and soft tissue infections.[4] The infection from S. putrefaciens most commonly involves skin and soft tissue associated with damage to skin (trauma, cut, ulcer) and otitis media. Primary bacteremia with fulminant course is also seen in immunocompromised patients. S. putrefaciens does not commonly cause lower respiratory tract infection. Respiratory colonization with the possibility of infection has been identified in rare cases from isolates cultured from sputum and pleura and transthoracic needle aspiration cultures.[5]

Both S. putrefaciens and Shewanella algae are uncommon isolates from clinical subjects, their natural habitats being all form of water, fish, oily food stuffs, and soils.[6],[7],[8]

CDC recognizes two biotypes of S. putrefaciens based on the requirement of sodium chloride for growth, oxidation of sucrose and maltose, and the ability to grow on Salmonella Shigella agar. Khashe and Janda have reported that S. algae is the predominant human clinical isolate while S. putrefaciens (CDC biotype 1) represents majority of nonclinical isolates.[9]

Nevertheless, S. putrefaciens also retains pathogenic potential, mainly under special environmental circumstances. Indeed, most of the reported infections are associated to contact with contaminated waters or injuries or occurrences in which integrity of the skin was compromised to some extent.[7],[10] In our case also, this might be source of infection. Potential incriminating agents are also long-term catheters. In addition, isolation of S. putrefaciens occurred in polymicrobial infections,[10] but in our case, it was the single pathogen isolated from blood culture.


   Conclusion Top


Although Shewanella is generally susceptible to antimicrobial agents, early diagnosis and prompt treatment can reduce morbidity and mortality among immunocompromised patients. Hence, a high level of suspicion is necessary. We conclude that these pathogens should not be considered as laboratory contaminants and should not be discarded as such.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Fredrickson JK, Zachara JM, Kennedy DW, Dong H, Onstott TC, Hinman NW, et al. Biogenic iron mineralization accompanying the dissimilatory reduction of hydrous ferric oxide by a groundwater bacterium. Geochim Cosmochim Acta 1998;62:3239-57.  Back to cited text no. 1
    
2.
Pagani L, Lang A, Vedovelli C, Moling O, Rimenti G, Pristerà R, et al. Soft tissue infection and bacteremia caused by Shewanella putrefaciens. J Clin Microbiol 2003;41:2240-1.  Back to cited text no. 2
    
3.
Hau HH, Gralnick JA. Ecology and biotechnology of the genus Shewanella. Annu Rev Microbiol 2007;61:237-58.  Back to cited text no. 3
    
4.
Durdu B, Durdu Y, Güleç N, Islim F, Biçer M. A rare cause of pneumonia: Shewanella putrefaciens. Mikrobiyol Bul 2012;46:117-21.  Back to cited text no. 4
    
5.
Jorens PG, Goovaerts K, Ieven M. Shewanella putrefaciens isolated in a case of ventilator-associated pneumonia. Respiration 2004;71:199-201.  Back to cited text no. 5
    
6.
Brink AJ, van Straten A, van Rensburg AJ. Shewanella (Pseudomonas) putrefaciens bacteremia. Clin Infect Dis 1995;20:1327-32.  Back to cited text no. 6
    
7.
Chen YS, Liu YC, Yen MY, Wang JH, Wang JH, Wann SR, et al. Skin and soft-tissue manifestations of Shewanella putrefaciens infection. Clin Infect Dis 1997;25:225-9.  Back to cited text no. 7
    
8.
Vogel BF, Jørgensen K, Christensen H, Olsen JE, Gram L. Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis. Appl Environ Microbiol 1997;63:2189-99.  Back to cited text no. 8
    
9.
Khashe S, Janda JM. Biochemical and pathogenic properties of Shewanella alga and Shewanella putrefaciens. J Clin Microbiol 1998;36:783-7.  Back to cited text no. 9
    
10.
Butt AA, Figueroa J, Martin DH. Ocular infection caused by three unusual marine organisms. Clin Infect Dis 1997;24:740.  Back to cited text no. 10
    

Top
Correspondence Address:
Priti Chowdhary
Department of Microbiology, ESI-PGIMSR, Basaidarapur, New Delhi- 110 015
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/IJPM.IJPM_254_16

Rights and Permissions


    Figures

  [Figure 1]



 

Top
 
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
   Introduction
   Case Report
   Discussion
   Conclusion
    References
    Article Figures

 Article Access Statistics
    Viewed1668    
    Printed25    
    Emailed0    
    PDF Downloaded57    
    Comments [Add]    

Recommend this journal