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  Table of Contents    
CASE REPORT  
Year : 2018  |  Volume : 61  |  Issue : 2  |  Page : 278-280
Extranodal histiocytic sarcoma in a child with acute lymphoblastic leukemia: Cytomorphological features of a rare entity with brief review of literature


1 Department of Pathology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
2 Department of Radiotherapy, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India

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Date of Web Publication20-Apr-2018
 

   Abstract 


Histiocytic sarcoma (HS) is a rare malignant neoplasm showing morphologic and immunophenotypic evidence of histiocytic differentiation. We describe a rare case of synchronous HS in a patient of B-cell acute lymphoblastic leukemia (ALL). A 16-year-old boy diagnosed as ALL also presented with a swelling over the right Achilles tendon. The cytological features of the swelling suggested a histiocytic lesion. Histological and immunohistochemical examination clinched the diagnosis of HS. The available 5-year follow-up showed no recurrence. It was a diagnostic dilemma on fine-needle aspiration. We discuss the cytological features of HS which can help in reaching a diagnosis and emphasize that it should be considered in the differential diagnosis for unexplained swellings in patients of hematological malignancies. Wide local excision of localized HS is associated with a long-term favorable outcome.

Keywords: Acute lymphoblastic leukemia, histiocytic sarcoma, synchronous

How to cite this article:
Pani KC, Yadav M, Kumar S, Agrawal V. Extranodal histiocytic sarcoma in a child with acute lymphoblastic leukemia: Cytomorphological features of a rare entity with brief review of literature. Indian J Pathol Microbiol 2018;61:278-80

How to cite this URL:
Pani KC, Yadav M, Kumar S, Agrawal V. Extranodal histiocytic sarcoma in a child with acute lymphoblastic leukemia: Cytomorphological features of a rare entity with brief review of literature. Indian J Pathol Microbiol [serial online] 2018 [cited 2020 Apr 9];61:278-80. Available from: http://www.ijpmonline.org/text.asp?2018/61/2/278/230562





   Introduction Top


Histiocytic sarcoma (HS) is a rare malignant neoplasm showing morphologic and immunophenotypic evidence of histiocytic differentiation.[1] It is defined in the WHO classification as a neoplastic proliferation with features of histiocytes. Extramedullary myeloid tumors with monocytic differentiation and dendritic cell neoplasms are excluded from the study. HS can be sporadic or may be clonally related to a hematologic malignancy, usually follicular lymphoma, or acute lymphoblastic leukemia (ALL).[1],[2] Cytological diagnosis is difficult unless a possibility of HS is considered in the differential diagnosis. We describe the pathological features of a rare case of HS involving the right Achilles tendon in a patient of ALL.


   Case Report Top


A 16-year-old boy presented with episodes of low-grade fever, diarrhea, and weight loss for 1 month. Physical examination revealed generalized lymphadenopathy and a small nodular painless swelling on the right Achilles tendon. Peripheral smear, bone marrow examination, and immunophenotyping were done, and the patient was diagnosed as B-cell ALL. Myeloperoxidase cytochemistry and immunophenotyping for myeloid markers were negative. He was put on standard risk BFM-90 protocol. Complete remission was achieved. The swelling over the Achilles tendon persisted. Further evaluation of the swelling was not performed, and the patient was on routine follow-up.

Five years later, the patient complained of increase in size of the right Achilles tendon swelling. Local examination revealed an ulceroproliferative growth measuring 5 × 4 × 4 which was fixed to the tendon. Computed tomography scan showed no involvement of the underlying bone. Fine-needle aspiration cytology (FNAC) and subsequent wide local excision of the growth with flap reconstruction were done at the local site. There was no evidence of recurrence of ALL on peripheral smear and bone marrow examination.

The FNAC smears were cellular and showed a dispersed population of large-to-medium-sized cells along with a mixed inflammatory cell infiltrate. The cells had round-to-oval eccentric nuclei, vesicular chromatin, 1–2 conspicuous nucleoli, and abundant pale to finely vacuolated cytoplasm. Few binucleate and multinucleate histiocytic cells along with some showing cytoplasmic debris and erythrophagocytosis were also seen [Figure 1]. Possibility of a histiocytic lesion was kept, and histopathology was advised for confirmation.
Figure 1: Cellular smears displaying large-to-medium-sized histiocytic cells along with a mixed inflammatory cell infiltrate. Few binucleate and multinucleate histiocytic cells showing vacuolization and erythrophagocytosis (May-Grunwald-Giemsa, ×200)

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Histology of the excised lesion showed sheets of polymorphic medium-to-large-sized cells with round-to-irregular vesicular nuclei and abundant eosinophilic cytoplasm along with interspersed mature lymphocytes, plasma cells, and occasional eosinophils. No nuclear grooving was seen. Focal spindling of the tumor cells, few multinucleated giant cells, hemophagocytic activity, and few mitotic figures were also seen [Figure 2]. On immunohistochemistry, the tumor cells showed expression of CD68, CD45, vimentin, and S-100 and were negative for CD1a, cytokeratin, CD30, CD10, CD117, CD34, HMB-45, and myogenin [Figure 3]. A diagnosis of HS involving the Achilles tendon was thus offered. The patient was on follow-up.
Figure 2: Histology section showing sheets of histiocytes with interspersed inflammatory cells with focal spindling (H and E, ×200)

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Figure 3: Strong cytoplasmic positivity for CD68 (×400)

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Five years later, the patient was diagnosed to have multiple space-occupying lesions in the right lobe of the liver. Ultrasound-guided FNAC, biopsy, and immunohistochemistry of liver lesions were performed. The results were consistent with leukemic infiltrate, rather than a histiocytic lesion (leukocyte common antigen, CD99 positive). Peripheral blood and bone examination done showed no relapse of leukemia. The patient was again started on high-dose chemotherapy and he responded very well to chemotherapy. The lesion on the Achilles tendon did not recur. However, 6 months later, the patient died of pancytopenia and severe sepsis.


   Discussion Top


HS is a hematopoietic neoplasm with morphological and immunohistochemical features of mature tissue histiocytes. It was described previously as “true histiocytic lymphoma” or as “malignant histiocytoses;” both of the terms are no longer used.[1] HS has been reported in association with malignant neoplasms, including a number of hematological malignancies.[1],[3] The other histiocytic lesions described in ALL are juvenile xanthogranulomas, Langerhans cell histiocytosis, Langerhans cell sarcoma, and Rosai–Dorfman disease.[4]

The risk factors for HS are unknown. Several earlier studies have reported histiocytic lesions in association with ALL. Soslow et al. described three cases of true HS in association with ALL.[5] There are studies which have performed molecular or cytogenetic analysis to establish the clonality between concurrent lymphoid and histiocytic neoplasms.[4],[6] These findings suggest the possibility of transdifferentiation of the two neoplasms which are morphologically and immunophenotypically distinct.[7] In our patient, the primary diagnostic consideration for the Achilles tendon mass was leukemic deposit. However, the cytological, histopathological, and immunohistochemical features of neoplastic cells were not compatible with that of lymphoblasts and favored a histiocytic proliferation.

HS can present as a solitary mass involving single organ or can present as a systemic disease. It can be nodal or extranodal. Gastrointestinal tract, soft tissue, and skin are the most common sites of extranodal involvement; however, uncommon sites such as salivary glands, breasts, lungs, pancreas, kidney, uterus, and central nervous system can be involved.[1],[2],[7],[8]

Studies on the cytological features of HS are sparse. Few earlier case reports have reported similar cytological features in HS as found in our patient.[9],[10] The cytological features described include medium-to-large cells with central-to-eccentric pleomorphic nuclei, vesicular chromatin, prominent nucleoli, and abundant pale to vacuolated cytoplasm associated with a prominent inflammatory cell infiltrate.[9],[10] The histopathological findings of HS are well documented and include diffuse noncohesive proliferation of round-to-oval large atypical cells with abundant eosinophilic cytoplasm. The tumor cells usually have oval-to-irregular nuclei with vesicular chromatin and prominent eosinophilic nucleoli. Binucleate or multinucleate forms are commonly seen. Variable mitotic activity, necrosis, and hemophagocytosis may be seen. Some tumors may contain a prominent stromal inflammatory infiltrate.[1],[2] By definition, expression of one or more histiocytic markers including CD68, lysozyme, CD163, or CD11c is necessary for diagnosis of HS. The tumor cells do not express B-cell, T-cell, or myeloid markers. Dendritic cell, epithelial, and melanocytic neoplasm markers should also be excluded from the study.[1],[2] Consistent cytological, histological, and immunohistochemical features were observed in our case.

Traditionally, HS is considered as an aggressive neoplasm and is associated with poor prognosis. A subset of patients who present with clinically localized disease treated with surgery alone has a favorable long-term outcome. Recurrence after wide excision can occur, especially in larger tumors.[1],[3],[8] Our patient had localized HS at presentation with no recurrence for a follow-up of 11 years till the patient died of sepsis.


   Conclusion Top


HS, though uncommon, can be associated with ALL. It should be considered in differential diagnosis of unexplained swellings in patients of hematological malignancies. FNAC is an important first-line diagnostic tool for suspecting the diagnosis and for guiding further management. Wide local excision of localized HS can be associated with recurrence-free long-term favorable outcome.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Grogan TM, Pileri SA, Chan JK, Weiss LM, Fletcher CD. Histiocytic sarcoma. In: Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, et al., editors. World Health Organization Classification of Tumours, WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. 4th ed. Lyon: International Agency for Research on Cancer (IARC); 2008. p. 356-7.  Back to cited text no. 1
    
2.
Takahashi E, Nakamura S. Histiocytic sarcoma: An updated literature review based on the 2008 WHO classification. J Clin Exp Hematop 2013;53:1-8.  Back to cited text no. 2
    
3.
Wongchanchailert M, Laosombat V. True histiocytic lymphoma following acute lymphoblastic leukemia. Med Pediatr Oncol 2003;40:51-3.  Back to cited text no. 3
    
4.
Castro EC, Blazquez C, Boyd J, Correa H, de Chadarevian JP, Felgar RE, et al. Clinicopathologic features of histiocytic lesions following ALL, with a review of the literature. Pediatr Dev Pathol 2009;30:1.  Back to cited text no. 4
    
5.
Soslow RA, Davis RE, Warnke RA, Cleary ML, Kamel OW. True histiocytic lymphoma following therapy for lymphoblastic neoplasms. Blood 1996;87:5207-12.  Back to cited text no. 5
    
6.
Kumar R, Khan SP, Joshi DD, Shaw GR, Ketterling RP, Feldman AL, et al. Pediatric histiocytic sarcoma clonally related to precursor B-cell acute lymphoblastic leukemia with homozygous deletion of CDKN2A encoding p16INK4A. Pediatr Blood Cancer 2011;56:307-10.  Back to cited text no. 6
    
7.
Onciu M. Histiocytic proliferations in childhood. Am J Clin Pathol 2004;122 Suppl:S128-36.  Back to cited text no. 7
    
8.
Hornick JL, Jaffe ES, Fletcher CD. Extranodal histiocytic sarcoma: Clinicopathologic analysis of 14 cases of a rare epithelioid malignancy. Am J Surg Pathol 2004;28:1133-44.  Back to cited text no. 8
    
9.
Tani E, Liliemark J, Svedmyr E, Mellstedt H, Biberfeld P, Skoog L, et al. Cytomorphology and immunocytochemistry of fine needle aspirates from blastic non-hodgkin's lymphomas. Acta Cytol 1989;33:363-71.  Back to cited text no. 9
    
10.
Miliauskas JR. Fine-needle aspiration cytology: True histiocytic lymphoma/histiocytic sarcoma. Diagn Cytopathol 2003;29:233-5.  Back to cited text no. 10
    

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Correspondence Address:
Vinita Agrawal
Department of Pathology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow - 226 014, Uttar Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/IJPM.IJPM_67_16

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    Figures

  [Figure 1], [Figure 2], [Figure 3]

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