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Year : 2019  |  Volume : 62  |  Issue : 1  |  Page : 159-162
Three unusual cases of parasites in eye


Department of Pathology, Dhruv Pathology and Molecular Diagnostic Lab, Nagpur, Maharashtra, India

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Date of Web Publication31-Jan-2019
 

   Abstract 


A 17-year-old male patient presented with cellulitis and mass in the eye noticed approximately 3 months back. The mass was about 1 cm in size and situated at the limbus. All preoperative routine investigations were normal. Surgical exploration revealed a sub-conjunctival cystic mass near the lateral rectus muscle about 1 cm in diameter; the mass was excised. Gross pathological examination revealed a thin-walled cystic mass. There was a hard nodule in the center. Microscopy revealed a wall of cysticercosis. Scolex was also seen. Surrounding tissue revealed sparse acute and chronic inflammatory cells. The case was confirmed by CDC, Atlanta, and was also included in their departmental presentation as an interesting case. A 60-year-old lady presented with complaints of itching over the forehead and right eye for 5 days. She was prescribed steroid eyedrops and antihistaminics. The itching aggravated with eyedrops along with watering and foreign body sensation. On revisit, the ophthalmologist noticed a worm in the right upper subconjunctival space. The worm was carefully removed in toto and sent to the laboratory for identification. The worm was thin, cylindrical, 8–10 cm long and white in color. After microscopic and gross examination of the worm, it was identified as Dirofilaria spp. CDC (Atlanta) confirmed the diagnosis of Dirofilaria. The patient was treated with antihistaminics and was relieved of symptoms without recurrence. A 45-year-old male patient had a painless mass in the eye for the last 3 months. He had no systemic illness. He gave a history of swimming pool use during that time. The mass was excised and submitted for histopathology. Numerous globular cysts representing thick-walled sporangia containing numerous spores diagnostic of Rhinosporidiosis were seen.

Keywords: Cysticercosis, dirofilaria, ophthalmic, parasite in eye, rhinosporidiosis

How to cite this article:
Mundhada S, Deshmukh M, Changole M. Three unusual cases of parasites in eye. Indian J Pathol Microbiol 2019;62:159-62

How to cite this URL:
Mundhada S, Deshmukh M, Changole M. Three unusual cases of parasites in eye. Indian J Pathol Microbiol [serial online] 2019 [cited 2019 Sep 22];62:159-62. Available from: http://www.ijpmonline.org/text.asp?2019/62/1/159/251243





   Introduction Top


Tinea solium, Dirofilaria, and Rhinosporidiosis parasitic infections are not so commonly seen. Moreover, eye is an unusual location for any parasite to harbor. The clinical presentation was also unusual. Three cases of these unusual parasites at an unusual location with an unusual presentation are detailed.


   Case Reports Top


Case 1: A rare case of isolated extraocular cysticercosis

Taeniasis is a zoonotic infections caused by cystodes or tapeworms named Tinea. There are two species of Tinea namely T. saginata and T. solium causing tineasis. They are called as cyclo- zoonoses because they require more than one vertebrate host to complete their developmental cycle (no invertebrate host).[1]T. saginata is caused by eating infected beef and is virtually present worldwide. Tapeworm infections caused by T. solium are more prevalent in underdeveloped communities with poor sanitation and where people eat raw or undercooked pork. Higher rates of illness have been seen in people in Latin America, Eastern Europe, sub-Saharan Africa, India, and Asia.[2]

A 17-year-old young male patient presented with complaints of redness and swelling around the right eye for 10 days. He gave a history of noticing a small mass in the right eye for three months. The mass was painless and growing very slowly. There was no watering of eyes, itching, or foreign body sensation. There was no diplopia, loss of vision, or other visual disturbances. There was no history of fever. No history of trauma/foreign body entering the eye was reported. The patient was a daily wager working in fields. He had no previous history of refractive errors or squint, ocular surgery, or ocular trauma. There was no history of tuberculosis, diabetes, or hypertension. He had no history of travel or exposure to pets.

On examination, vision in both eyes was 6/6 and fundus was normal. Anterior segment examination in the left eye was normal. Anterior segment examination of the right eye revealed conjunctival congestion and chemosis temporally. It also revealed soft, nontender, irreducible extraocular nodule of approximately 1 cm in size. Rest of the anterior segment and fundus examination was normal. Ocular movements were normal. Ultrasonography B scan revealed a thin-walled cystic lesion around the limbus near the lateral rectus muscle. The mass was removed surgically and sent for histopathological examination.

Gross examination of the mass revealed a cystic grayish-white mass of approximately 1 cm in size. The mass was thin-walled and on opening there was a hard nodule in the center of the cavity.

Microscopy showed a lamellar, eosinophilic wall, typical of cysticercosis. Presence of scolex and calcareous capsule confirmed the diagnosis of cysticercosis. There were sparse inflammatory cells in the surrounding. The microscopic pictures sent to Center for Disease Control (CDC), Atlanta through their web portal service reconfirming the diagnosis.

Neurological examination and Computed Tomography (CT) of the brain was done to rule out neurocysticercosis, but both were negative. Other systemic involvement was also ruled out for cysticercosis. The patient received antihelminthic treatment in the form of Tab. Albendazole 400 mg for 1 month. Follow-up after 1 month was negative with no signs and symptoms of reinfection [Figure 1].
Figure 1: Gross and microscopy of cysticercosis. Eosinophillic membranous wall with scolex seen

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Case 2: An unusual case of Dirofilaria in eye

Dirofilariasis is a group of parasitoses caused by the genus Dirofilaria transmitted by vectors to domestic and wild animals. D. immitis and D. nochtiella repens are the most common Dirofilaria due to their severe pathological effects and their high incidence and prevalence.[3]D. immitis produces both canine and feline cardiopulmonary dirofilariasis, whereas D. repens causes both canine and feline subcutaneous dirofilariasis.[4],[5] Similarly, in humans, D. immitis and D. repens are responsible for pulmonary and subcutaneous/ocular dirofilariasis, respectively, throughout the world.[4],[5]

A 60-year-old housewife presented with itching over the forehead and right eye for 5 days. There was no history of headache, nausea, vomiting, or any systemic complaints. There was no history of any recent ophthalmic intervention. There was no history of contact with animals or recent travel. There was no history of allergy, asthma, hypertension, tuberculosis, or diabetes mellitus.

On examination, vision in both eyes was 6/6 and fundus was normal. Local examination of the right eye revealed normal anterior chamber. Patient was prescribed steroid eye drops and antihistaminics (Fexofenadine 180 mg/day). Her itching aggravated with instillation of eye drops. Patient revisited the outpatient department with continuous watering from the right eye. Severe foreign body sensation and photophobia were also present. At this time, thorough examination of the right eye revealed a worm in the upper sub-conjunctival area. The worm was removed completely under local anesthesia. There was immediate relief from symptoms and the patient was discharged on local and oral antihistaminics. Further treatment was not necessitated in this case as only a single worm was present. Patient is asymptomatic since this episode.

Gross and microscopy (specimen identification)

The worm was received in toto in the laboratory. It was white, thin, cylindrical, and approximately 8–10 cm long. Gross and light microscopy examination led to the diagnosis of Dirofilaria spp.

The diagnosis of Dirofilaria spp. was reconfirmed based on gross and microscopic photographs sent to CDC, Atlanta through their web portal service [Figure 2].
Figure 2: Anterior end of Dirofilaria immitis showing oesophagus (×100), Uterus and ova (×200)

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Case 3. An unusual case of Rhinosporidiosis in eye

A 45-year-old male patient presented with a painless mass in the left eye for the last 3 months. He had no systemic illness. He gave a history of swimming pool use during that time. The mass was excised and submitted for histopathology. Microscopic examination revealed numerous globular cysts representing thick walled sporangia containing numerous spores diagnostic of Rhinosporidiosis [Figure 3].
Figure 3: Gross and microscopic appearance with spores of Rhinosporidiosis

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   Discussion Top


Case 1

Infection with T. solium tapeworms can result in human cysticercosis, which can be a very serious disease if the brain or eye gets involved. Humans are the definitive hosts for T. solium with pigs being the intermediate host.[1] Infection occurs through infective eggs in undercooked pork. Eggs or gravid proglottids are passed with feces. These mature eggs contaminate pastures and barnyards, where cattle and pigs ingest them. Upon reaching the alimentary canal of infected animals, the embryos are released, penetrating the gut wall and entering the circulation and organs. Humans develop a tapeworm infection by eating raw or undercooked pork.

Cysticercosis is the development of extraintestinal encysted larval forms of T solium in various organs.[6] Their effects depend entirely upon the location of the cysticerci. Most common sites are brain and subcutaneous tissue sometimes involving the eye, liver, etc. Ocular cysticercosis may affect any tissue of the eye either extraocular (subconjunctival or orbital tissues) or intraocular (vitreous, subretinal space, or anterior chamber). The ocular manifestations can vary from asymptomatic in early stages to rather destructive later on because lesions gradually increase in size leading to blindness in 3–5 years.[7] Bilateral involvement is rare but multiple lesions may develop in the same eye.[8],[9] Intraocular cysticercosis usually occurs as a part of systemic infection. Isolated ocular involvement is uncommon in clinical practice.[10] Basic laboratory investigations may reveal eosinophilia in complete blood count (CBC). Enzyme-linked immunosorbent assay (ELISA) detecting anti-cysticercal antibodies may be helpful in suspected cases. Radiological diagnosis in the form of ultrasonography B scan and CT scan are more reliable in the detection and determining the extent of disease. Fine needle aspiration cytology (FNAC) may be helpful sometimes. Histopathological examination should be routinely done after cyst removal. Surgical removal is mandatory in ocular cysticercosis.[11] Cysts deep within the orbit are best treated conservatively with a 4-week regimen of oral Albendazole (15 mg/kg/d) in conjunction with oral steroids (1.5 mg/kg/d) in a tapering dose over a 1-month period.[12] Ocular cysticercosis is a rare and preventable cause of blindness. High level of suspicion along with use of appropriate diagnostic modalities is very crucial in the management of isolated ocular cysticercosis.

Case 2

Dogs and a wide variety of other animals such as beavers and horses are definitive hosts for Dirofilaria. Aedes, Culex mosquitoes are the vectors for Dirofilaria. An infected mosquito introduces third-stage filarial larvae of Dirofilaria into the skin of the definitive host.[13] Humans are not suitable hosts for Dirofilaria species but only accidental hosts as they do not play a role in the propagation of life cycle. Immature D. immitis worms can reach a branch of the human pulmonary artery and trigger an inflammatory response. This may occasionally result in pulmonary nodules. The worms usually do not grow to maturity. D. immitis larvae tend to follow the same migratory pathway as in the canine host, ending up in the lungs, where they often lodge in small vessels causing infarcts and typical “coin lesions” visible on radiographs.[13] In India, human ocular dirofilarial infections have been reported from Kerala in 1976 and 1978 initially.[14] Later on, subconjunctival dirofilariasis due to D. repens were reported from several parts of India. The identification of the worm is done by studying the morphology of the fully matured worm both macroscopically and microscopically. Dirofilaria are identified by their thick laminated cuticle, broad lateral ends, and large muscle cells.[15],[16]D. immitis can be differentiated from D. repens by absence of ridges. Surgical removal of the worm is one of the modalities of treatment in Dirofilaria infections.[17] D. immitis causes microfilaremia in humans and may require the use of antihelmenthic drugs.

The reporting of human dirofilariasis in general along with ocular dirofilariasis has dramatically increased since the last few years. This might be attributed to many factors including climate change leading to altered vector behavior and steep rise in travelling across the globe. Hence, diagnosing this emerging parasitic infection should not be accidental only. A high index of suspicion on the part of ophthalmologists/clinicians and knowledge about morphologic features of the worm by microbiologists should give us an accurate insight into incidence of dirofilariasis.

Case 3

Rhinosporidiosis is common in the nose, eye being relatively rare. The disease spreads from animal to humans. In a series of 49 cases, in Kerala they could trace a majority of infection to a pond.[18]

Declaration of patient consent

The authors certify that they have obtained all appropriate patient consent forms. In the form the patient(s) has/have given his/her/their consent for his/her/their images and other clinical information to be reported in the journal. The patients understand that their names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Park K. Taeniasis. Park's textbook of Preventive and Social Medicine. 23rd ed. Bhanot Publishers; 2017.p.302-3.  Back to cited text no. 1
    
2.
Available from: http://www.cdc.gov/parasites/taeniasis/health_professionals/index.html. [Last accessed on 2018 Jun 18].  Back to cited text no. 2
    
3.
Simón F, Siles-Lucas M, Morchón R, González-Miguel J, Mellado I, Carretón E,et al. Human and Animal Dirofilariasis: The Emergence of a Zoonotic Mosaic. Clin Microbiol Rev 2012;25:507-44.  Back to cited text no. 3
    
4.
Pampiglione S, Canestri Trotti G, Rivasi F. Human dirofilariasis due to Dirofilaria (Nochtiella) repens: A review of world literature. Parasitology 1995;37:149-93.  Back to cited text no. 4
    
5.
Simón F, López-Belmonte J, Marcos-Atxutegi C, Morchón R, Martín- Pacho JR. What is happening outside North America regarding human dirofilariasis? Vet Parasitol 2005;133:181-9.  Back to cited text no. 5
    
6.
Available from: https://emedicine.medscape.com/article/999727-overview/Tinea infections. [Last accessed on 2018 Jun 18].  Back to cited text no. 6
    
7.
Sharma T, Sinha S, Shah N, Gopal L, Shanmugam MP, Bhende P,et al. Intraocular cysticercosis: Clinical characteristics and visual outcome after vitreoretinal surgery. Ophthalmology 2003;110:996-1004.  Back to cited text no. 7
    
8.
Topilow HW, Yimoyines DJ, Freeman HM, Young GA, Addison R. Bilateral multifocal intraocular cysticercosis. Ophthalmology 1981;88:1166-72.  Back to cited text no. 8
    
9.
Kapoor S, Kapoor MS. Ocular cysticercosis. J Pediatr Ophthalmol Strabismus 1978;15:170-3.  Back to cited text no. 9
    
10.
Rahman M, Brahma N, Bhanu Devi, Kuli JJ. Isolated Ocular Cysticercosis-Case Report. IOSR J Dent Med Sci 2015;14:25-7.  Back to cited text no. 10
    
11.
Pushker N, Bajaj MS, Chandra M, Neena. Ocular and orbital cysticercosis. Acta Ophthalmol Scand 2001;79:408-13.  Back to cited text no. 11
    
12.
Kaur A, Agrawal A, Agrawal PK, Goel MM. Lacrimal canalicular obstruction by cysticercus cellulosae. Orbit 2006;25:163-5.  Back to cited text no. 12
    
13.
Available from: http://www.cdc.gov/parasites/dirofilariasis/biology_d_immitis.html. [Last accessed on 2018 Jun 18].  Back to cited text no. 13
    
14.
Padmaja P, Kanagalakshmi, Samuel R, Kuruvilla PJ, Mathai E. Subcutaneous dirofilariasis in Southern India: A case report. Ann Trop Med Parasitol 2005;99:437-40.  Back to cited text no. 14
    
15.
Kini RG, Leena JB, Shetty P, Hart R, Sumanth LD, George L. Human dirofilariasis: An emerging zoonosis in India. J Parasit Dis 2015;39:349-54.  Back to cited text no. 15
    
16.
Achappa B, Madi D, Mahalingam S. An interesting case of subcutaneous nodule. J Clin Diagn Res 2013;7:364-5.  Back to cited text no. 16
    
17.
Nath R, Gogoi R, Bordoloi N, Gogoi T. Ocular dirofilariasis. Indian J Pathol Microbiol 2010;53:157-9.  Back to cited text no. 17
[PUBMED]  [Full text]  
18.
Suseela V, Subramaniam KS. Rhinosporidiosis and the Eyes. Indian J Ophthalmol 1975;23:1-4.  Back to cited text no. 18
[PUBMED]  [Full text]  

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Correspondence Address:
Shailendra Mundhada
Dhruv Pathology and Molecular Diagnostic Lab, 3rd Floor, Aditya Enclave, 20A Central Bazar Road, Ramdas Peth, Nagpur - 440 010, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/IJPM.IJPM_247_18

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