Indian Journal of Pathology and Microbiology


Year
: 1995  |  Volume : 38  |  Issue : 3  |  Page : 261--5

Bauhinia purpurea and Pisum sativum lectin binding in human breast.


A B Sarker, T R Koirala, I Murakami, H J Jeon 
 Department of Pathology, Okayama University Medical School, Japan

Correspondence Address:
A B Sarker
Department of Pathology, Okayama University Medical School, Japan

The binding of biotinylated bauhinia purpurea (BPA) and pisum Sativum (PSA) lectins to paraffin-embedded tissue sections from 10 normal breast and 55 breast carcinoma has been investigated by applying avidin biotin peroxidase method (ABC). BPA showed very low affinity for normal breast epithelium and the binding was confined to the luminal surface. Eighty-seven percent carcinoma bound BPA, and the staining patterns varied depending on the histologic grade of tumors: luminal surface binding in grade 1 carcinomas; diffuse, granular cytoplasmic with para- or perinuclear deposits and staining along the plasma membrane in grade 2 and grade 3 carcinomas. PSA bound consistently to the luminal surface of all ducts and acinar cells of normal breast tissue. PSA was reactive with all carcinoma but the staining profiles were similar regardless of the tumor differentiation. It is concluded that the lectins used in this study have limited usefulness in routine diagnosis.


How to cite this article:
Sarker A, Koirala T, Murakami I, Jeon H. Bauhinia purpurea and Pisum sativum lectin binding in human breast. Indian J Pathol Microbiol 1995;38:261-5


How to cite this URL:
Sarker A, Koirala T, Murakami I, Jeon H. Bauhinia purpurea and Pisum sativum lectin binding in human breast. Indian J Pathol Microbiol [serial online] 1995 [cited 2020 May 28 ];38:261-5
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=1995;volume=38;issue=3;spage=261;epage=5;aulast=Sarker;type=0