Indian Journal of Pathology and Microbiology


Year
: 1995  |  Volume : 38  |  Issue : 3  |  Page : 299--303

Characterization of mycobacterial antigens by Elisa and immunoblot methods.


A Mathai, V V Radhakrishnan, S Sehgal 
 Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum

Correspondence Address:
A Mathai
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum

Antibodies to two mycobacterial antigens viz - culture filtrate antigen (CFA) and Mycobacterium tuberculosis antigen 5 were raised in rabbits. Enzyme-linked immuno sorbent assay (ELISA) and immunoblot methods were used for the evaluation of the specificity of the rabbit antibodies to M. tuberculosis. Immunoblot method is more sensitive than ELISA for the detection of antibodies to M. tuberculosis in the rabbit sera. It is being emphasised that characterisation of the mycobacterial antigens and evaluation of the specificity of the antimycobacterial antibodies are essential prior to their applications as an adjunct in the laboratory diagnosis of human mycobacterial disease.


How to cite this article:
Mathai A, Radhakrishnan V, Sehgal S. Characterization of mycobacterial antigens by Elisa and immunoblot methods. Indian J Pathol Microbiol 1995;38:299-303


How to cite this URL:
Mathai A, Radhakrishnan V, Sehgal S. Characterization of mycobacterial antigens by Elisa and immunoblot methods. Indian J Pathol Microbiol [serial online] 1995 [cited 2020 May 26 ];38:299-303
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=1995;volume=38;issue=3;spage=299;epage=303;aulast=Mathai;type=0