Indian Journal of Pathology and Microbiology


Year
: 1996  |  Volume : 39  |  Issue : 1  |  Page : 13--7

Role of immunophenotyping in characterisation of blast crisis of chronic myeloid leukemia--a study of 25 cases.


V Padmanabhan, N Varma, S Dash, S Varma 
 Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
V Padmanabhan
Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

The blast cell populations of 25 patients of chronic myeloid leukemia in blast crisis (CML-BC) were studied for morphological, cytochemical and immunophenotypic features. The patients were divided into 6 broad groups based upon the pattern of surface marker positivity-myeloblastic, mixed myeloblastic, megakaryoblastic, mixed lineage, lymphoid and undifferentiated blast crisis. Myeloperoxidase (MPO), Sudan Black B (SBB) and Chloroacetate esterase (CAE) stains showed 100% specificity for the myelomonocytic lineage but the sensitivity was low. Periodic acid Schiff (PAS) stain was neither specific nor sensitive for the lymphoid lineage. Immunophenotyping as compared to morphologic and cytochemical assessment, was seen to be most useful for assigning a lineage to leukemic cells in CML-BC.


How to cite this article:
Padmanabhan V, Varma N, Dash S, Varma S. Role of immunophenotyping in characterisation of blast crisis of chronic myeloid leukemia--a study of 25 cases. Indian J Pathol Microbiol 1996;39:13-7


How to cite this URL:
Padmanabhan V, Varma N, Dash S, Varma S. Role of immunophenotyping in characterisation of blast crisis of chronic myeloid leukemia--a study of 25 cases. Indian J Pathol Microbiol [serial online] 1996 [cited 2020 Jul 3 ];39:13-7
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=1996;volume=39;issue=1;spage=13;epage=7;aulast=Padmanabhan;type=0