Indian Journal of Pathology and Microbiology


Year
: 1997  |  Volume : 40  |  Issue : 3  |  Page : 361--4

Fish meal extract bile esculin agar (FMBE) a selective medium for Bacteroides fragilis group.


V K Beena, S Rao, M Kotian, P G Shivananda 
 Department of Microbiology, Kasturba Medical College, Manipal

Correspondence Address:
V K Beena
Department of Microbiology, Kasturba Medical College, Manipal

Fish meal extract bile esculin agar (FMBE) is prepared using Fish meal extract concentrate as the basal substance, for the selective isolation and presumptive identification of B.fragilis group. The efficiency of the medium was evaluated by growing stock cultures of B.fragilis groups as well as inoculating clinical specimens and comparing the results with Bacteroides bile esculin agar (BBE). All the 87 stock cultures of B.fragilis grew on FMBE and BBE. No other anaerobes tested grew on the medium. However 7 out of 65 neomycin resistant aerobes grew on the FMBE. From the 100 clinical samples, 62 strains of B. Fragilis group were recovered on FMBE and BBE, and 53 strains on supplemented BHIBA. The cost effectiveness, selectivity and the ability to detect esculin hydrolysis will enable FMBE as a suitable medium as comparable to that of BBE, if not superior.


How to cite this article:
Beena V, Rao S, Kotian M, Shivananda P. Fish meal extract bile esculin agar (FMBE) a selective medium for Bacteroides fragilis group. Indian J Pathol Microbiol 1997;40:361-4


How to cite this URL:
Beena V, Rao S, Kotian M, Shivananda P. Fish meal extract bile esculin agar (FMBE) a selective medium for Bacteroides fragilis group. Indian J Pathol Microbiol [serial online] 1997 [cited 2019 Aug 24 ];40:361-4
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=1997;volume=40;issue=3;spage=361;epage=4;aulast=Beena;type=0