Indian Journal of Pathology and Microbiology


Year
: 2001  |  Volume : 44  |  Issue : 2  |  Page : 97--102

Polymerase chain reaction using IS6110 primer to detect Mycobacterium tuberculosis in clinical samples.


P Gunisha, H N Madhavan, U Jayanthi, K L Therese 
 Vision Research Foundation, Sankara Nethralaya, 18, College Road, Chennai

Correspondence Address:
P Gunisha
Vision Research Foundation, Sankara Nethralaya, 18, College Road, Chennai

Nucleic acid amplification using IS6110 primers to detect Mycobacterium tuberculosis in clinical specimens has been extensively used as laboratory tool for the diagnosis for tuberculosis. Despite itSQs dramatic scientific value in practice, it is not as sensitive as expected for the detection of M. tuberculosis. The results of the study suggest that PCR using 123 bp fragment of DNA belonging to IS6110 is specific (95.6%) but only has a sensitivity of 30% to detect M. tuberculosis in clinical specimens.


How to cite this article:
Gunisha P, Madhavan H, Jayanthi U, Therese K. Polymerase chain reaction using IS6110 primer to detect Mycobacterium tuberculosis in clinical samples. Indian J Pathol Microbiol 2001;44:97-102


How to cite this URL:
Gunisha P, Madhavan H, Jayanthi U, Therese K. Polymerase chain reaction using IS6110 primer to detect Mycobacterium tuberculosis in clinical samples. Indian J Pathol Microbiol [serial online] 2001 [cited 2019 Aug 25 ];44:97-102
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2001;volume=44;issue=2;spage=97;epage=102;aulast=Gunisha;type=0