Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2008  |  Volume : 51  |  Issue : 4  |  Page : 497--499

Detection of extended spectrum beta-lactamase from clinical isolates in Davangere


PN Sridhar Rao, KG Basavarajappa, G Leela Krishna 
 Department of Microbiology, JJM Medical College, Davangere 577 004, Karnataka, India

Correspondence Address:
P N Sridhar Rao
Department of Microbiology, JJM Medical College, Davangere 577 004, Karnataka
India

Extended spectrum β-lactamase enzymes (ESBLs) are enzymes that have the ability to hydrolyze oxyiminocephalosporins and infections by isolates producing them are often difficult to treat. A study to detect the presence of these enzymes in isolates was conducted by our hospital. A total of 207 non repetitive isolates were screened for resistance to any of five screening agents. Those with suspicious profiles were checked for ESBL production by double-disk approximation or a synergy test. The isolates were also subjected to a phenotypic confirmation test as recommended by CLSI (formerly NCCLS). Various cephalosporins-β-lactamase inhibitor combinations were also tested. Of the 204 (98.5%) screen-positive isolates, only 126 (61.7%) were identified as ESBL producers. Of these, 26.1% of the isolates were positive by using the double-disk synergy test (DDST) method alone, 13.4% were positive using the method recommended by CLSI, and 60.3% of the isolates were positive by both the DDST and CLSI methods. We also report a high percentage of resistance to cefoxitin (96.8%) indicating changes in porins.


How to cite this article:
Sridhar Rao P N, Basavarajappa K G, Krishna G L. Detection of extended spectrum beta-lactamase from clinical isolates in Davangere.Indian J Pathol Microbiol 2008;51:497-499


How to cite this URL:
Sridhar Rao P N, Basavarajappa K G, Krishna G L. Detection of extended spectrum beta-lactamase from clinical isolates in Davangere. Indian J Pathol Microbiol [serial online] 2008 [cited 2020 Mar 30 ];51:497-499
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2008;volume=51;issue=4;spage=497;epage=499;aulast=Sridhar;type=0