Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2012  |  Volume : 55  |  Issue : 3  |  Page : 314--318

A novel tissue microarray instrumentation:The HT-1 tissue microarrayer


Jun Yang1, Mingjuan Zhang2, Baoshan Su1, XiaoLi Chen1, AnJing Kang1 
1 Department of Pathology, Second Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China
2 Department of Cardiology, Second Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China

Correspondence Address:
Jun Yang
Department of Pathology, Second Hospital of Medical College, Xi«SQ»an Jiaotong University, 157 West 5th Road, Xi«SQ»an, Shaan«SQ»xi 710004
China

Background: Tissue microarray (TMA) is a novel and useful tool to efficiently analyze gene expression in histological tissues. Aim: Cost-efficient and easy to use automated tissue arrayers will provide a better instrumentation to generate TMAs. Thus, we designed and produced our tissue microarrayer to meet these needs. Materials and Methods: The HT-1 tissue microarrayer we designed and manufactured consists primarily of four parts, including an instrument to make array pores for the recipient paraffin blocks, a punch needle, an instrument for negative-pressure embedding, and a special manipulator. By using the HT-1, 14 different TMAs were made to accommodate 312 cases of tissues and TMA sections were tested by hematoxylin-eosin (H&E) staining, in situ hybridization, and immunohistochemistry. Results: Expand: Hematoxylin and eosin staining showed that the tissue cylinders were similar, even, and in order on the slides. Most importantly, the HT-1 microarrayer can make array pores in the recipient paraffin block with a single application in seconds. The HT-1 also contains a unique negative pressure system for embedding TMA blocks. In addition, HT-1 can make tissue cylinders with the same levels and depth for equally embedded and sectioning. Conclusions: The HT-1 tissue microarrayer is a device that is simple, economical and easy to use.


How to cite this article:
Yang J, Zhang M, Su B, Chen X, Kang A. A novel tissue microarray instrumentation:The HT-1 tissue microarrayer.Indian J Pathol Microbiol 2012;55:314-318


How to cite this URL:
Yang J, Zhang M, Su B, Chen X, Kang A. A novel tissue microarray instrumentation:The HT-1 tissue microarrayer. Indian J Pathol Microbiol [serial online] 2012 [cited 2019 Nov 15 ];55:314-318
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=3;spage=314;epage=318;aulast=Yang;type=0