Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2012  |  Volume : 55  |  Issue : 4  |  Page : 481--484

Correlation between HER2 gene amplification and protein overexpression through fluorescence in situ hybridization and immunohistochemistry in breast carcinoma patients


RN Makroo1, Mohit Chowdhry1, Manoj Kumar1, Priyanka Srivastava1, Richa Tyagi1, Preeti Bhadauria1, Sumaid Kaul2, Ramesh Sarin3, PK Das4, Harsh Dua4 
1 Transplant Immunology, Molecular Biology and Transfusion Medicine, Apollo Hospitals, Sarita Vihar, Delhi Mathura Road, New Delhi, India
2 Department of Histopathology, Apollo Hospitals, Sarita Vihar, Delhi Mathura Road, New Delhi, India
3 Department of Surgical Oncology, Apollo Hospitals, Sarita Vihar, Delhi Mathura Road, New Delhi, India
4 Department of Medical Oncology, Apollo Hospitals, Sarita Vihar, Delhi Mathura Road, New Delhi, India

Correspondence Address:
Mohit Chowdhry
Transplant Immunology, Molecular Biology and Transfusion Medicine, Apollo Hospitals, Sarita Vihar, Delhi Mathura Road, New Delhi
India

Background : In India, the incidence of breast cancer has increased in the urban population, with 1 in every 22 women diagnosed with breast cancer. It is important to know the HER2/neu gene status for a better prognostication of these patients. Aim : The aim of this study was to compare the efficacy of fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) for determining HER2/neu alteration in breast carcinoma. Materials and Methods : A total of 188 histologically proven breast carcinoma cases between the years 2007 and 2011 were retrospectively analyzed on the paraffin tissue sections by both IHC and FISH techniques. FISH for HER2/neu gene amplification was performed on cases where the IHC status was already known and the results were compared. Results : A total of 64 (30%) patients were found to be amplified and the remaining 124 (65.9%) cases were found to be unamplified through FISH. Patients observed with 3+ reading on IHC were later confirmed as unamplified in 29.5% cases through FISH. Conclusion : It has been confirmed with the present study that IHC is a prudent first-step technique to screen tissue samples for HER2/neu gene status, but should be supplemented with the FISH technique especially in equivocal cases.


How to cite this article:
Makroo R N, Chowdhry M, Kumar M, Srivastava P, Tyagi R, Bhadauria P, Kaul S, Sarin R, Das P K, Dua H. Correlation between HER2 gene amplification and protein overexpression through fluorescence in situ hybridization and immunohistochemistry in breast carcinoma patients.Indian J Pathol Microbiol 2012;55:481-484


How to cite this URL:
Makroo R N, Chowdhry M, Kumar M, Srivastava P, Tyagi R, Bhadauria P, Kaul S, Sarin R, Das P K, Dua H. Correlation between HER2 gene amplification and protein overexpression through fluorescence in situ hybridization and immunohistochemistry in breast carcinoma patients. Indian J Pathol Microbiol [serial online] 2012 [cited 2020 Jul 14 ];55:481-484
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=4;spage=481;epage=484;aulast=Makroo;type=0