Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 57  |  Issue : 4  |  Page : 537--541

Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India


Meher Rizvi1, Mohd Azam1, Asfia Sultan1, Indu Shukla1, Abida Malik1, Masihur Reman Ajmal2, Fatima Khan1, Hiba Sami1 
1 Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University (AMU), Aligarh, Uttar Pradesh, India
2 Department of Medicine, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University (AMU), Aligarh, Uttar Pradesh, India

Correspondence Address:
Asfia Sultan
Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University (AMU), Aligarh, Uttar Pradesh
India

Background: Etiology of nearly 30% cases of chronic viral hepatitis remains undetected. Occult HBV infection (OBI) has emerged as an important clinical entity in this scenario. Apart from prevalence and clinical outcome of OBI patients genotype was determined in northern region of India. Materials and Methods: A total of 847 patients with chronic liver disease (CLD) were screened for common viral etiologies and others serological markers of HBV. Amplification of surface, precore and polymerase genes of HBV was performed in patients negative for other etiologies. Genotyping and sequencing of the precore region was performed for OBI cases. Results: Twenty-nine (7.61%) cases of OBI were identifiedof which 9 had chronic liver disease (CHD), 11 liver cirrhosis (LC) and 9 hepatocellular carcinoma (HCC). Majority of OBI cases were detected by amplification of surface gene 26 (89.6%), followed by pre-core gene 12 (41.3%). Their liver functions tests were significantly deranged in comparison to overt HBV cases. IgG anti HBc was present in 8 (27.6%) OBI cases. Mutation was observed in 8 (32%) in pre-core region at nt. 1896 of overt HBV cases. Genotype D was the predominant genotype. In conclusion: OBI in our study was characterized by predominance of genotype D and more severe clinical and biochemical profile in comparison to overt HBV. IgG anti HBc positivity could be utilized as a marker of OBI. We recommend use of sensitive nested PCR for diagnosis of OBI, amplifying at least surface and precore gene.


How to cite this article:
Rizvi M, Azam M, Sultan A, Shukla I, Malik A, Ajmal MR, Khan F, Sami H. Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India .Indian J Pathol Microbiol 2014;57:537-541


How to cite this URL:
Rizvi M, Azam M, Sultan A, Shukla I, Malik A, Ajmal MR, Khan F, Sami H. Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India . Indian J Pathol Microbiol [serial online] 2014 [cited 2020 Aug 4 ];57:537-541
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2014;volume=57;issue=4;spage=537;epage=541;aulast=Rizvi;type=0