Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 59  |  Issue : 3  |  Page : 314--317

Comparative analysis of phenotypic and genotypic detection of methicillin resistance among Staphylococcus aureus


Tulin Demir1, Nilay Coplu2, Berrin Esen3 
1 Department of Clinical Microbiology, Ahi Evran University Research and Training Hospital, Kirsehir; Department of Communicable Diseases Control Programs, Public Health Institution, National Microbiology Reference Laboratories, Ankara, Turkey
2 Department of Clinical Microbiology, Ministry of Health, Dıskapı Research and Training Hospital, Ankara, Turkey
3 Department of Clinical Microbiology, Ankara Research and Training Hospital, Ankara, Turkey

Correspondence Address:
Dr. Tulin Demir
Public Health Institution, National Microbiology Reference Laboratories, Ankara
Turkey

Aims: Staphylococcus aureus is a common pathogen causing a wide range of infections ranging from mild skin and soft tissue infections to severe, life-threatening infections. Accuracy in the detection of methicillin resistance is important to avoid treatment failures. The aim of this study was to compare the results of phenotypic and genotypic test methods to detect methicillin resistance and also to determine the antimicrobial susceptibilities. Materials and Methods: Two hundred and forty-two S. aureus strains isolated from skin and soft tissue samples were analyzed for methicillin resistance using oxacillin and cefoxitin disk diffusion (DD), oxacillin screen agar test, cefoxitin E-test, and mecA gene polymerase chain reaction (PCR). Results: 77 of 242 S. aureus isolates were mecA positive. Oxacillin, cefoxitin DD, oxacillin screen agar test and cefoxitin E-test exhibited sensitivities as 98.7%, 98.7%, 100%, 100%, and specificities as 96.9%, 97.5%, 96.9%, 97.5%, respectively. Conclusion: Results of oxacillin screen agar and cefoxitin DD test were in concordance with mecA gene PCR. Thus, it is determined that especially cefoxitin test can be an alternative to PCR in routine.


How to cite this article:
Demir T, Coplu N, Esen B. Comparative analysis of phenotypic and genotypic detection of methicillin resistance among Staphylococcus aureus.Indian J Pathol Microbiol 2016;59:314-317


How to cite this URL:
Demir T, Coplu N, Esen B. Comparative analysis of phenotypic and genotypic detection of methicillin resistance among Staphylococcus aureus. Indian J Pathol Microbiol [serial online] 2016 [cited 2020 Sep 22 ];59:314-317
Available from: http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2016;volume=59;issue=3;spage=314;epage=317;aulast=Demir;type=0