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  Indian J Med Microbiol
 

Figure 1: The immunity identification of recombination Sap2 (rSap2). Protein was separated by 12% sodium dodecyl sulfate — polyacrylamide gel electrophoresis and was transferred onto a nitrocellulose membrane. Immunized serum, negative serum, candidiasis serum and normal serum were used to react with protein. The results showed that the recombinant protein can be recognized by antibody in immunized serum and candidiasis serum. However, no antibody in negative serum and normal serum can react with rSap2. Lane 1, marker; lane 2, rSap2 protein against candidiasis serum; lane 3, rSap2 against normal serum; lane 4, rSap2 protein against immunized serum; lane 5, rSap2 protein against negative serum

Figure 1: The immunity identification of recombination Sap2 (rSap2). Protein was separated by 12% sodium dodecyl sulfate — polyacrylamide gel electrophoresis and was transferred onto a nitrocellulose membrane. Immunized serum, negative serum, candidiasis serum and normal serum were used to react with protein. The results showed that the recombinant protein can be recognized by antibody in immunized serum and candidiasis serum. However, no antibody in negative serum and normal serum can react with rSap2. Lane 1, marker; lane 2, rSap2 protein against candidiasis serum; lane 3, rSap2 against normal serum; lane 4, rSap2 protein against immunized serum; lane 5, rSap2 protein against negative serum