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  Indian J Med Microbiol
 

Figure 6: The loss of chromosomes 9 and 18 were observed in only tumor ti ssue; see Figure 2A and B using molecular inversion probe array The probe value = 2 is copy number neutral, probe value <1.5 is loss and that >2.5 is gain. Figure 2A shows the loss of chromosome 18 and 2B shows the loss of chromosome 9. Figure 2C and D are fl uorescent in situ hybridizati on (FISH) analyses for chromosomes 9 and 18 using the LSI probe for the CDKN2A locus at 9p21 in spectrum Orange and centromeric probes CEP 9 in spectrum Green and CEP 18 (D18Z1) in spectrum Aqua. Figure 2C is FISH from the normal ti ssue; two Orange, two Green and two Aqua signals detected, indicating disomy for chromosomes 9 and 18. Figure 2D is FISH for tumor ti ssue, which has only one Orange, one Aqua and one Green signal, indicati ng a loss of chromosomes 9 and 18

Figure 6: The loss of chromosomes 9 and 18 were observed in only tumor ti ssue; see Figure 2A and B using molecular inversion probe array The probe value = 2 is copy number neutral, probe value <1.5 is loss and that >2.5 is gain. Figure 2A shows the loss of chromosome 18 and 2B shows the loss of chromosome 9. Figure 2C and D are fl uorescent in situ hybridizati on (FISH) analyses for chromosomes 9 and 18 using the LSI probe for the CDKN2A locus at 9p21 in spectrum Orange and centromeric probes CEP 9 in spectrum Green and CEP 18 (D18Z1) in spectrum Aqua. Figure 2C is FISH from the normal ti ssue; two Orange, two Green and two Aqua signals detected, indicating disomy for chromosomes 9 and 18. Figure 2D is FISH for tumor ti ssue, which has only one Orange, one Aqua and one Green signal, indicati ng a loss of chromosomes 9 and 18