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  Indian J Med Microbiol
 

Figure 1: Ewing sarcoma (a-d). (a) Plain radiograph: diaphyseal, permeative lesion, involving fibula with layered periosteal reaction. (b) Microscopy: malignant small round cells in a diffuse pattern (H and E, ×400). (c) By immunohistochemistry (IHC), tumor cells displaying diffuse, intense cytoplasmic membranous staining for CD99/MIC2 (Diaminobenzidine, ×400). (d) Diffuse intranuclear Fli1 immunoreactivity (Diaminobenzidine, ×400). (e) Focal immunostaining synaptophysin positivity (Diaminobenzidine, ×400)

Figure 1: Ewing sarcoma (a-d). (a) Plain radiograph: diaphyseal, permeative lesion, involving fibula with layered periosteal reaction. (b) Microscopy: malignant small round cells in a diffuse pattern (H and E, ×400). (c) By immunohistochemistry (IHC), tumor cells displaying diffuse, intense cytoplasmic membranous staining for CD99/MIC2 (Diaminobenzidine, ×400). (d) Diffuse intranuclear Fli1 immunoreactivity (Diaminobenzidine, ×400). (e) Focal immunostaining synaptophysin positivity (Diaminobenzidine, ×400)