Indian Journal of Pathology and Microbiology
Home About us Instructions Submission Subscribe Advertise Contact e-Alerts Ahead Of Print Login 
Users Online: 2572
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
Year : 1998  |  Volume : 41  |  Issue : 2  |  Page : 157-62

Application of lactophenol cotton blue for identification and preservation of intestinal parasites in faecal wet mounts.

Department of Microbiology, Rural Medical College and Pravara Rural Hospital, Maharashtra

Correspondence Address:
H Khubnani
Department of Microbiology, Rural Medical College and Pravara Rural Hospital, Maharashtra

Login to access the Email id

Source of Support: None, Conflict of Interest: None

PMID: 9670627

Rights and PermissionsRights and Permissions

Wet mounting is the simplest and easiest technique for the examination of faeces. Routinely employed wet mounts in most of the laboratories include saline, iodine and buffered methylene blue. Lactophenol cotton blue [LCB] staining--mounting medium is commonly used for microscopic identification of fungi. LCB and its modifications were tested as staining mounting medium on parasite positive faecal samples [both fresh as well as formalin fixed] to stain and demonstrate morphology and to preserve parasitic elements [trophozoites, cysts, ova and larvae] in wet mounts. Protozoal cysts [Entamoeba histolytica and Giardia lamblia], helminthic OVA [Ancylostoma duodenale, Ascaris lumbricoides, Enterobius vermicularis, Taenia solium and Hymenolepis nana] and larvae [Strongyloides stercoralis] were well-stained and preserved their morphology in nailpolish enamel sealed LCB-faecal mounts. Morphology was better appreciated in LCB-formalin fixed faecal mounts as compared to LCB fresh faecal mounts and was well preserved by the end of twelve weeks.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded135    
    Comments [Add]    

Recommend this journal