Indian Journal of Pathology and Microbiology
Home About us Instructions Submission Subscribe Advertise Contact e-Alerts Ahead Of Print Login 
Users Online: 425
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
Year : 2007  |  Volume : 50  |  Issue : 4  |  Page : 733-9

The effect of different DNA isolation methods on the outcome of high-risk HPV testing.


Department of Pathology, Stavanger University Hospital, Stavanger, Norway

Correspondence Address:
Irene T Oevestad
Department of Pathology, Stavanger University Hospital, Stavanger, Norway

Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 18306539

Rights and PermissionsRights and Permissions

High-risk human papillomavirus (hr-HPV) testing is widely used, but different DNA isolation methods may give variable results in PCR determinations. To evaluate the effect of different DNA isolation methods on the HPV results, DNA was isolated from 180 consecutive cervical samples with the manual Amplilute kit, or the standard automated or a modified MagNAPure LC (MPLC) protocol. The DNA was then tested for hr-HPV with the AMPLICOR HPV-test, and the HPV-genotype was determined with the Linear Array (LA) test. The concordance of the three DNA isolation methods is 90-93% and 88-92% between the HPV-tests from DNA isolated by any of the three isolation methods and LA. The sensitivity, specificity, percentage of false negatives and false positives compared to the LA detected hrHPV subtypes are similar for the manual and standard MPLC, but the specificity of the modified MPLC was much lower and the percentage of false positives much higher. In conclusion both the manual and standard MPLC DNA isolation methods give good HPV results. The standard MPLC is the fastest DNA isolation method.


[PDF Not available]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1094    
    Printed78    
    Emailed0    
    PDF Downloaded1    
    Comments [Add]    
    Cited by others 1    

Recommend this journal