Indian Journal of Pathology and Microbiology
Home About us Instructions Submission Subscribe Advertise Contact e-Alerts Ahead Of Print Login 
Users Online: 399
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
Year : 2008  |  Volume : 51  |  Issue : 1  |  Page : 108-112

Comparative study of peripheral blood smear, quantitative buffy coat and modified centrifuged blood smear in malaria diagnosis

1 Department of Pathology, Kasturba Medical College, Mangalore, India
2 Department of Community Medicine, Kasturba Medical College, Mangalore, India
3 Head of Pathology, A J Institute of Medical Science, Mangalore, India

Correspondence Address:
P L Bhandari
"Mangala", Chilimbi Hilldale Road, Ladyhill, P.O. Ashoknagar, Mangalore - 575 006, Karnataka
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0377-4929.40419

Rights and Permissions

The present study was aimed at modifying the centrifuged blood smear (modified centrifuged blood smear or MCBS), to make it a feasible and standardized procedure. The results obtained were compared with the current diagnostic methods - peripheral blood smear (PBS) and quantitative buffy coat (QBC). Blood samples collected from 100 suspected malaria patients were subjected to all three tests. It was found that PBS had 86.79% sensitivity and was absolutely specific. QBC was 96.22% sensitive and 93.61% specific. The majority of variations occurred in PBS negative cases; cases with parasite count <++ and with Plasmodium falciparum. It was seen that by the addition of centrifugation to the conventional smear technique (MCBS) improved its sensitivity from 86.79% to near 100%. QBC and MCBS were found superior to PBS. Since MCBS combines principles of both QBC and PBS, it is as sensitive as QBC, as specific as PBS, and above all, easily performed and affordable.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded1199    
    Comments [Add]    
    Cited by others 13    

Recommend this journal