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Year : 2012  |  Volume : 55  |  Issue : 4  |  Page : 610-611
Evaluation of immunochromatographic test in early serological diagnosis of dengue fever

1 Department of Pathology and Microbiology, Sir Ronald Ross Institute of Tropical and Communicable Diseases, Hyderabad, Andhra Pradesh, India
2 Department of Microbiology, Osmania Medical College, Hyderabad, Andhra Pradesh, India

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Date of Web Publication4-Mar-2013

How to cite this article:
Kalyani D, Bai M M. Evaluation of immunochromatographic test in early serological diagnosis of dengue fever. Indian J Pathol Microbiol 2012;55:610-1

How to cite this URL:
Kalyani D, Bai M M. Evaluation of immunochromatographic test in early serological diagnosis of dengue fever. Indian J Pathol Microbiol [serial online] 2012 [cited 2021 Nov 28];55:610-1. Available from: https://www.ijpmonline.org/text.asp?2012/55/4/610/107863


Early detection of dengue fever is the need of the hour as it is currently the most important mosquito-borne viral disease affecting humans.

Presently, antibody detection by enzyme-linked immunosorbent assay (ELISA) is the most efficacious method for diagnosis of dengue fever. [1] However, due to the time factor and significant false positive and false negative results, this method has its own limitations. But Immunochromatographic test (ICT) for the simultaneous detection of IgG and IgM is unhindered by the limitations of a conventional method. [1] As IgM antibody appears early in course of disease, its detection by ICT is a valuable tool in the rapid diagnosis than conventional ELISA method.

The main objective of this hospital based study is to compare the efficacy of ICT with the conventional ELISA. This study was done on 100 patients who were admitted with fever and low platelet count in Sir Ronald Ross institute of tropical and communicable diseases, Hyderabad during the months of September and October 2011.

Patient's data including age, sex, clinical signs and symptoms, family history were noted. All the routine radiological and hematological tests were done for each case. For all cases, conventional ELISA was done, while both conventional ELISA and ICT were done for 40 samples that included both normal and abnormal results by the conventional method.

Selection of 40 cases was randomly done and subjected to availability of rapid test kits. Rest of the cases were used as controls.

We observed that ICT showed high positivity rate in 22.5% (9 out of 40 cases) compared to conventional ELISA that showed 5% positivity (2 out of 40 cases) as shown in [Table 1]. 22% (7 out of 40 samples) samples which were positive on ICT were negative on conventional ELISA. None of the ICT negative samples were positive on conventional ELISA. Both ICT and ELISA could be done only for 40 out of 100 cases due to the disrupted supply of ICT kits.
Table 1: Comparative positivity of samples on enzyme-linked immunosorbent assay and Immunochromatographic test

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This is a rapid (<10 min) ICT for detection of both dengue-specific IgM and IgG in a test card format. No pre-treatment of sera to remove competing IgG or rheumatoid factor was required. Preparation and dilution of reagents along with multiple incubation steps as in Blots could be avoided. It is easily adapted as the reagents and instrumentation are widely available. Similar studies concluded that ICT is the simplest and the fastest assay. [2],[3],[4]

To conclude, ICT is found to be highly sensitive when compared to conventional ELISA for serological diagnosis of Dengue fever. As it is easy to perform, needs no expertise and elaborate equipment and an early reporting of results is possible, thereby facilitating and an early diagnosis.

   Acknowledgments Top

The authors wish to thank Dr. Suryaprabha, Professor, Department of Social and Preventive Medicine, Osmania Medica College, Hyderabad for her help in statistical evaluation of this study.

   References Top

1.Sathish N, Vijayakumar TS, Abraham P, Sridharan G. Dengue fever: Its laboratorydiagnosis, with special emphasis on IgM detection 116. Dengue Bulletin 2003;27:116-25.  Back to cited text no. 1
2.Vaughn DW, Nisilak A, Kalayanarooj S, Solomon T, Dung NM, Cuzzubbo A, Devine PL. Evaluation of a rapid immunochromatographic test for diagnosis of dengue virus infection. J Clin Microbiol 1998;36:234-8.  Back to cited text no. 2
3.Sang CT, Hoon LS, Cuzzubbo A, Devine P. Clinical evaluation of a rapid immunochromatographic test for the diagnosis of dengue virus infection. Clin Diagn Lab Immunol 1998;5:407-9.  Back to cited text no. 3
4.Groen J, Koraka P, Velzing J, Copra C, Osterhaus AD. Evaluation of six immunoassays for detection of dengue virus-specific immunoglobulinM and G antibodies. Clin Diagn Lab Immunol 2000;7:867-71.  Back to cited text no. 4

Correspondence Address:
D Kalyani
Department of Pathology, Sir Ronald Ross Institute of Tropical and Communicable Diseases, Hyderabad, Andhra Pradesh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0377-4929.107863

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