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Year : 2019  |  Volume : 62  |  Issue : 2  |  Page : 319-322
Non-Hodgkin's lymphoma with extensive emperipolesis mimicking Rosai–Dorfman disease: A rare case report

Department of Pathology, Byramjee Jeejeebhoy Government Medical College and Sassoon General Hospital, Pune, Maharashtra, India

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Date of Web Publication10-Apr-2019


Emperipolesis is the hallmark finding for Rosai-Dorfman disease. Till now many studies in literatures have shown emperipolesis as a finding in other benign as well as malignant conditions. Very few cases of malignant lymphoma have this phenomenon. Herewith, we put forward a rare case of lymphoma with clinical presentation showing involvement of spleen, liver, lymph nodes as well as lleo-cecal region. Light microscopy revealed large to medium sized lymphoid cells with intervening plenty of histiocytes showing evidence of emperipolesis that mimics Rosai Dorfmann disease. Due to atypical clinical presentations we thought of lymphoma as a differential diagnosis. Further immunohistochemistry was performed using histiocytic as well as lymphoid markers. To our surprise, it turns out to be Non Hodgkin Lymphoma with extensive emperipolesis which is extremely rare in thorough literature search. This case is presented due to its unique clinical as well as histological presentations.

Keywords: Emperipolesis, immunohistochemistry, non-Hodgkin's lymphoma, Rosai–Dorfman disease

How to cite this article:
Rane SR, Parkhi M, Vishwasrao S, Nakate L. Non-Hodgkin's lymphoma with extensive emperipolesis mimicking Rosai–Dorfman disease: A rare case report. Indian J Pathol Microbiol 2019;62:319-22

How to cite this URL:
Rane SR, Parkhi M, Vishwasrao S, Nakate L. Non-Hodgkin's lymphoma with extensive emperipolesis mimicking Rosai–Dorfman disease: A rare case report. Indian J Pathol Microbiol [serial online] 2019 [cited 2022 Oct 7];62:319-22. Available from:

   Introduction Top

In 1956, Humble et al. were the first to describe the term “emperipolesis” that includes the presence of a viable intact cell or cells within the cytoplasm of another cell.[1] Since then, it has been reported in several conditions such as Rosai–Dorfman disease (RDD) (most commonly showing numerous intact lymphocytes engulfed by the histiocytes within their cytoplasm), idiopathic thrombocytopenic purpura, autoimmune hemolytic anemia, multiple myeloma, leukemia, malignant lymphoma, carcinoma, neuroblastoma, and giant cell carcinoma of lung.[2],[3] Emperipolesis has also been described in cases of inflammatory myxohyaline tumor[4] and myelodysplastic syndrome.[5] Emperipolesis in association with lymphoma is rare, with only few cases reported in the literature; most of them are based on tissue culture specimens, bone marrow aspirates, or cytology.[6]

RDD and lymphoma share significant overlapping clinical picture; however, they differ in their management protocol and outcome. Thus, it is of utmost important to be knowledgeable about the fact that emperipolesis does occur in a lymphoma.[7] In view of rare association between emperipolesis and lymphoma in contrast to RDD, we present a case of a non-Hodgkin's lymphoma (NHL) with extensive emperipolesis including morphologic and immunohistochemical studies.

   Case Report Top

A 52-year-old-male came to our hospital with abdominal lump and associated pain for the past 6–8 months. He complained of fever and loose motions for the past 6–7 days. In addition, the patient had a significant reduction in weight of 10 kg over a period of 6 months. History of breathlessness was also present. At the time of admission, the patient was febrile. General examination revealed pulse 110 beats/min, respiratory rate 24/min, and blood pressure 120/80 mmHg. Per abdominal examination, massive spleen was palpable which was extending beyond left coastal margin to reach below the umbilicus. Respiratory, cardiovascular, and central nervous systems' examination were within normal limits. The patient had a history of left-sided inguinal lymphadenopathy which was biopsied and reported outside as granulomatous lymphadenitis.

At the time of admission, his laboratory tests showed normochromic and normocytic anemic picture (hemoglobin = 10.9 g/dL) with mild thrombocytopenia. Erythrocyte sedimentation rate (30 mm at the end of 1 h) and lactic acid dehydrogenase (880 U/L) were elevated. Serum electrolytes, liver function tests, and total proteins were within normal limit. Chest X-ray was within normal limit. Electrocardiogram depicted sinus tachycardia. Computed tomography (CT) of abdomen revealed massive splenomegaly that showed heterogeneously enhanced areas predominantly in upper pole. Linear nonenhancing areas were also noted in mid and lower poles of spleen, likely to be infarcts. Caecum displayed asymmetrical wall thickening with mild perilesional fat stranding. Also seen was a large mass attached to the liver. Enlarged lymph nodes of variable sizes were noted in periportal, aortocaval, and precaval regions.

We received the right hemicolectomy specimen along with whole of spleen and part of the liver. The surgeon shared the intraoperative findings of a large peri-pancreatic lymph node which seems to be involved; however, it was not removed. Grossly, spleen was markedly enlarged measuring around 24 × 17 × 9 cm. The external surface was lobulated and congested. On serial slicing, there are multiple well-demarcated variable sized nodules that completely effaced normal splenic parenchyma. The cut surface appeared solid, homogeneous, yellow-white and firm, and showed large areas of hemorrhage [Figure 1]. Part of liver received was measuring 15 × 7.5 × 4.5 cm and showed presence of well-demarcated tumor with similar above gross findings and compressed normal liver parenchyma at periphery. Multiple varying sized nodular masses with similar above gross findings, largest measuring 9 × 6 × 5.5 cm, were noted in mesentery of the caecum which was seen reaching the mucosa causing large areas of ulceration. Multiple sections through tumor mass of all three specimens were studied and microscopically showed similar histopathological findings in the form of diffuse proliferation of medium to large sized neoplastic lymphoid cells having irregular nuclear membrane, vesicular nucleus, prominent nucleoli, and scant eosinophilic cytoplasm [Figure 2]a. Mitotic figures were high. Intermingled, there are numerous large histiocytes with vesicular nuclei, distinct nucleoli, and abundant pale cytoplasm showing characteristic lymphophagocytosis along with few engulfed red blood cells (emperipolesis). Liver sections revealed tumor cells infiltrating the surrounding liver parenchyma. In addition to tumor deposits, some foci of well-formed epithelioid cell granulomas were identified in one of the sections from caecum. On further work-up, normal serum ferritin level, hypercellular bone marrow with no hemophagocytosis, and absence of other criteria helped us to rule out hemophagocytic lymphohistiocytosis syndrome.
Figure 1: Spleen – on serial slicing there are multiple well-demarcated variable sized nodules that completely effaced normal splenic parenchyma. The cut surface appeared solid, homogeneous, yellow-white and firm, and showed large areas of hemorrhage

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Figure 2: (a) The photograph from spleen microscopically reveals diffuse proliferation of medium to large sized neoplastic lymphoid cells with intermingled histiocytes showing emperipolesis (better seen in the inset picture – 1000 × magnification). (b and c) These neoplastic lymphoid cells demonstrate membranous immunoreactivity for CD19 and CD20. (d) MUM1 immunostain displays nuclear positivity in 40%–50% of tumor cells. (e) Very focal BCL6 nuclear positivity in tumor cells is noted (<5%). (f) CD68-positive immunostain in histiocytes

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Immunohistochemistry performed on tissue sections showed that the neoplastic lymphoid cells were diffusely positive for CD19 (membranous) [Figure 2]b, CD20 (membranous) [Figure 2]c, and MUM1 (nuclear; 40%–50%) [Figure 2]d; focally positive for CD43 (membranous); and are negative for CD3, CD5, CD23, Bcl2, MPO, and EBV-LMP. Bcl6 immunostain showed very focal nuclear immunoreactivity (<5%) [Figure 2]e. Histiocytes showing emperopolesis were highlighted by CD68 [Figure 2]f. Ki-67 labeling index was 80%–90%. According to the Real European-American Lymphoma/World Health Organization 2017 classification (REAL/WHO 2017) and taking into account the clinical inputs, CT findings, histopathological, and immunohistochemistry examination, the tumor was classified as diffuse large B-cell lymphoma (DLBCL) and activated B-cell phenotype with extensive emperopolesis.

   Discussion Top

Emperipolesis, first described by Humble et al. in 1956, is a condition in which viable hematopoietic cells are seen in the cytoplasm of host cells that may be in the form of megakaryocyte, monocyte, endothelial cells, fibroblast, or malignant cells.[7] In contrast to phagocytosis, emperipolesis shows the absence of destruction of the engulfed cell and host cell. To our knowledge, Overholtzer et al. recently presented a new term called “entosis” that depicts a homogeneous cell-in-cell phenomenon and a striking similarity to emperipolesis.[8]

RDD, also known as sinus histiocytosis with massive lymphadenopathy, showed emperipolesis as a hallmark feature. This finding was not considered pathognomonic because it is also demonstrated in other conditions such as Hodgkin's disease, leukemia, acute and chronic myeloid leukemia, NHL, myeloproliferative disorders, myelodysplastic syndrome, multiple myeloma, neuroblastoma, and rhabdomyosarcoma.[5],[9] Some investigators are in the opinion that emperipolesis occurs as a result of an active adherence of lymphocytes to tumor cells or macrophages with a subsequent inclusion in vacuoles inside the cytoplasm of these cells.[1],[10]

Taking into account very few case reports in literature, emperipolesis and lymphoma shared rare association. In comparison to Hodgkin's lymphoma, emperipolesis has been noted more commonly in NHL. Not to forgot that most of these cases displayed emperipolesis in bone marrow aspirate, tissue culture studies, or cytology.[7]

In our case, the initial impression of RDD on hematoxylin and eosin sections was a mislead diagnosis on the basis of emperipolesis. As we know that RDD, a benign proliferative disorder of histiocytes, has an indolent clinical course with most of the patients undergoing complete resolution without any treatment. By performing immunohistochemistry, we come to know the immunophenotype of the lymphoma (DLBCL) showing extensive emperipolesis. Among few related case studies on tissue sections, Lopes et al. in 2003 showed a case of diffuse B-cell lymphoma with emperipolesis. Their results had the support of ancillary techniques such as immunohistochemistry and electron microscopy.[9]

Talking about the additional histopathological finding of epithelioid granulomas in one of the section of caecal mass and previous left inguinal lymph node, we think this could be a precursor lesion that must have lead to presentations of RDD which further turned to be lymphoma. This could be further substantiated properly using molecular studies AND ancillary technique.

   Conclusion Top

Lack of awareness of occurrence of emperipolesis phenomenon in a lymphoma leads to misdiagnosis and delay in management and treatment. Through our case and few previous related literatures, we just want to conclude that whenever a patient comes with such type of clinical presentation along with emperipolesis in lymphoid background on histopathology, always think of lymphoma with extensive emperipolesis as a mimicker to RDD and it should be further confirmed on immunohistochemistry.

Declaration of patient consent

The authors certify that they have obtained all appropriate patient consent forms. In the form the patient(s) has/have given his/her/their consent for his/her/their images and other clinical information to be reported in the journal. The patients understand that their names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.

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Conflicts of interest

There are no conflicts of interest.

   References Top

Humble JG, Jayne WH, Pulvertaft RJ. Biological interaction between lymphocytes and other cells. Br J Haematol 1956;2:283-94.  Back to cited text no. 1
Foucar E, Rosai J, Dorfman RF. Sinus histiocytosis with massive lymphadenopathy. Current status and future directions. Arch Dermatol 1988;124:1211-4.  Back to cited text no. 2
Djaldetti M, Strauss Z. Emperipolesis by megakaryocytes in patients with non-Hodgkin's lymphoma and megaloblastic anemia. J Submicrosc Cytol 1982;14:407-13.  Back to cited text no. 3
Kinkor Z, Mukensnabl P, Michal M. Inflammatory myxohyaline tumor with massive emperipolesis. Pathol Res Pract 2002;198:639-42.  Back to cited text no. 4
Sable MN, Sehgal K, Gadage VS, Subramanian PG, Gujral S. Megakaryocytic emperipolesis: A histological finding in myelodysplastic syndrome. Indian J Pathol Microbiol 2009;52:599-600.  Back to cited text no. 5
[PUBMED]  [Full text]  
Overholtzer M, Mailleux AA, Mouneimne G, Normand G, Schnitt SJ, King RW, et al. Anonapoptotic cell death process, entosis, that occurs by cell-in-cell invasion. Cell 2007;131:966-79.  Back to cited text no. 6
Lee KP. Emperipolesis of hematopoietic cells within megakaryocytes in bone marrow of the rat. Vet Pathol 1989;26:473-8.  Back to cited text no. 7
Poppema S. Sternberg-reed cells with intracytoplasmic lymphocytes. Phagocytosis or emperipolesis? Virchows Arch A Pathol Anat Histol 1978;380:355-9.  Back to cited text no. 8
Lopes LF, Bacchi MM, Coelho KI, Filho AA, Bacchi CE. Emperipolesis in a case of B-cell lymphoma: A rare phenomenon outside of Rosai-Dorfman disease. Ann Diagn Pathol 2003;7:310-3.  Back to cited text no. 9
Amita K, Vijay Shankar S, Abhishekh MG, Geethalakshmi U. Emperipolesis in a case of adult T cell lymphoblastic lymphoma (mediastinal type)-detected at FNAC and imprint cytology. Online J Health Allied Sci 2011;10:11.  Back to cited text no. 10

Correspondence Address:
Sharada R Rane
Department of Pathology, Byramjee Jeejeebhoy Government Medical College and Sassoon General Hospitals, Pune - 411 001, Maharashtra
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/IJPM.IJPM_409_18

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