Indian Journal of Pathology and Microbiology


Year
: 2000  |  Volume : 43  |  Issue : 3  |  Page : 343--6

Rapid identification of fluconazole resistance using Chromagar Candida.


S L Verghese, P Padmaja, P Sutha, T Mathew, E S John 
 Department of Microbiology, Institute of Cardio-Vascular Diseases, Chennai

Correspondence Address:
S L Verghese
Department of Microbiology, Institute of Cardio-Vascular Diseases, Chennai

Although extremely rare 10 years ago, antifungal drug resistance is becoming a major problem in certain populations, especially in those infected with HIV. This study was undertaken to study the resistance of Candida species isolated in our hospital to Fluconazole using Chrom agar Candida. The Candida strains which were routinely isolated from clinical specimens like blood, urine, sputum, pus, fluid and homograft isolates were included in the study. 142 Candida isolates were tested by using Chrom agar Candida incorporated with fluconazole. 16 strains were found to be resistant to Fluconazole and 126 strains sensitive to Fluconazole. Nine were C tropicalis, 3 C krusei, 2 C guillermondii, 1 Geotrichum candidum and one was an unidentified strain of Candida. The MIC of the 16 strains were done using RPMI 1640 medium by macro broth dilution method. MIC of 9 strains was 64 & > 64 micrograms/ml of 6 strains 32 micrograms/ml and 1 strain 16 micrograms/ml.


How to cite this article:
Verghese S, Padmaja P, Sutha P, Mathew T, John E. Rapid identification of fluconazole resistance using Chromagar Candida. Indian J Pathol Microbiol 2000;43:343-6


How to cite this URL:
Verghese S, Padmaja P, Sutha P, Mathew T, John E. Rapid identification of fluconazole resistance using Chromagar Candida. Indian J Pathol Microbiol [serial online] 2000 [cited 2021 Jan 22 ];43:343-6
Available from: https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2000;volume=43;issue=3;spage=343;epage=6;aulast=Verghese;type=0