Indian Journal of Pathology and Microbiology


Year
: 2002  |  Volume : 45  |  Issue : 1  |  Page : 75--7

Role of IgM specific indirect immunofluorescence assay in diagnosing an outbreak of leptospirosis.


Suvarna Joshi, Abhijit Bal, Renu Bharadwaj, Rameshwar Kumbhar, Anju Kagal, Vidya Arjunwadkar 
 Department of Microbiology, B J Medical College, Pune

Correspondence Address:
Suvarna Joshi
Department of Microbiology, B J Medical College, Pune

The study was conducted during a suspected epidemic of leptospirosis in Maharashtra. A total of 13 acute phase blood samples, collected at 5-6 days from the onset of symptoms, and 10 convalescent phase samples, colected at around 20 days from the onset of symptoms were obtained from 13 patients. Sera were separated and the samples were subjected to Microagglutination Test (MAT) and IgM-Indirect Immunofluorescence assay (IFA) to detect antibodies against leptospira. In the acute phase sera, only one sample was positive by MAT while seven were positive by IFA. In the convalescent samples, six were positive by MAT and seven were positive by IFA. IFA is a rapid test and can be used for early diagnosis of leptospirosis.


How to cite this article:
Joshi S, Bal A, Bharadwaj R, Kumbhar R, Kagal A, Arjunwadkar V. Role of IgM specific indirect immunofluorescence assay in diagnosing an outbreak of leptospirosis. Indian J Pathol Microbiol 2002;45:75-7


How to cite this URL:
Joshi S, Bal A, Bharadwaj R, Kumbhar R, Kagal A, Arjunwadkar V. Role of IgM specific indirect immunofluorescence assay in diagnosing an outbreak of leptospirosis. Indian J Pathol Microbiol [serial online] 2002 [cited 2021 Oct 23 ];45:75-7
Available from: https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2002;volume=45;issue=1;spage=75;epage=7;aulast=Joshi;type=0