Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 56  |  Issue : 1  |  Page : 20--23

Anti-Toxoplasma gondii antibody detection in serum and urine samples by enzyme-linked immunosorbent assay in HIV-infected patients


Sayan Bhattacharyya1, Sumeeta Khurana2, Mohan Lal Dubey2 
1 Department of Microbiology, KGMU, Lucknow, Uttar Pradesh, India
2 Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Sayan Bhattacharyya
481, P. Majumdar Road, Kolkata -700 078, West Bengal
India

Background: Toxoplasmosis is a common parasitic infection of man, and reactivation of latent disease in HIV-infected patients can cause fatal encephalitis. Diagnosis depends on demonstration of parasite-specific antibodies in serum. In HIV-infected patients, IgM is often undetectable, whereas IgG remains detectable in the majority. Urine sample is very easily available and has not been evaluated for immunodiagnosis of toxoplasmosis. Aim: The study was an effort to find whether urine sample can be used in place of serum for immunodiagnosis of toxoplasmosis. Materials and Methods: Enzyme-linked immunosorbent assay (ELISA) was carried out in serum and urine samples collected from 100 HIV-infected patients to detect anti-toxoplasma IgG and IgM antibodies and whether positivity correlated with the CD4 T-cell counts of patients. Results: In this study, we observed that there was no significant difference in positivity of anti-toxoplasma IgM and IgG between serum and urine samples of HIV-infected patients by ELISA. There was a negative correlation between CD4 count and seropositivity. Conclusion: Urine sample can be satisfactorily used in place of serum for immunodiagnosis of toxoplasmosis.


How to cite this article:
Bhattacharyya S, Khurana S, Dubey ML. Anti-Toxoplasma gondii antibody detection in serum and urine samples by enzyme-linked immunosorbent assay in HIV-infected patients.Indian J Pathol Microbiol 2013;56:20-23


How to cite this URL:
Bhattacharyya S, Khurana S, Dubey ML. Anti-Toxoplasma gondii antibody detection in serum and urine samples by enzyme-linked immunosorbent assay in HIV-infected patients. Indian J Pathol Microbiol [serial online] 2013 [cited 2021 Oct 27 ];56:20-23
Available from: https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2013;volume=56;issue=1;spage=20;epage=23;aulast=Bhattacharyya;type=0