Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 61  |  Issue : 2  |  Page : 214--218

Detection of immunoglobulin M and immunoglobulin G antibodies to Mycoplasma pneumoniae in children with community-acquired lower respiratory tract infections


Surinder Kumar1, Indu Bala Garg1, Gulshan Rai Sethi2, Sanchit Kumar1, Sanjeev R Saigal1 
1 Department of Microbiology, Maulana Azad Medical College, New Delhi, India
2 Department of Pediatrics, Maulana Azad Medical College, New Delhi, India

Correspondence Address:
Surinder Kumar
Department of Microbiology, Maulana Azad Medical College, New Delhi - 110 002
India

Context: Mycoplasma pneumoniae (M. pneumoniae) causes up to 40% of community-acquired pneumonia in children. It is impossible to identify M. pneumoniae infection on the basis of clinical signs, symptoms, and radiological features. Therefore, correct etiological diagnosis strongly depends on laboratory diagnosis. Aims: This study aims to investigate the role of M. pneumonia e in pediatric lower respiratory tract infections (LRTIs) employing enzyme-linked immunosorbent assays (ELISA) and particle agglutination (PA) test. Settings and Design: Two hundred and eighty children, age 6 months to 12 years with community-acquired LRTIs were investigated for M. pneumoniae etiology. Materials and Methods: We investigated 280 children hospitalized for community-acquired LRTIs, using ELISA and PA test for detecting M. pneumoniae immunoglobulin M (IgM) and immunoglobulin G antibodies. Statistical Analysis Used: The difference of proportion between the qualitative variables was tested using the Chi-square test and Fischer exact test. P ≤ 0.05 was considered as statistically significant. Kappa value was used to assess agreement between ELISA and PA test. Results: M. pneumoniae was positive in 51 (23.2%) <5 years and 33 (54.0%) children in ≥5 years of age group, and this difference was statistically significant (P < 0.001). Clinical and radiological findings in M. pneumoniae positive and negative groups were comparable. ELISA detected M. pneumoniae in 78 (27.8%) and PA test 39 (13.9%) patients; 33 (84.6%) ELISA positive and 6 (15.4%) ELISA negative. ELISA/PA test together detected M. pneumoniae infection in 84 (30%) children. Conclusions: Our data underline that M. pneumoniae plays an important role in children with community-acquired LRTIs and more particularly in children >5 years of age.


How to cite this article:
Kumar S, Garg IB, Sethi GR, Kumar S, Saigal SR. Detection of immunoglobulin M and immunoglobulin G antibodies to Mycoplasma pneumoniae in children with community-acquired lower respiratory tract infections.Indian J Pathol Microbiol 2018;61:214-218


How to cite this URL:
Kumar S, Garg IB, Sethi GR, Kumar S, Saigal SR. Detection of immunoglobulin M and immunoglobulin G antibodies to Mycoplasma pneumoniae in children with community-acquired lower respiratory tract infections. Indian J Pathol Microbiol [serial online] 2018 [cited 2021 Apr 20 ];61:214-218
Available from: https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2018;volume=61;issue=2;spage=214;epage=218;aulast=Kumar;type=0