Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 63  |  Issue : 4  |  Page : 521--526

Circulating free DNA as a marker of response to chemoradiation in locally advanced head and neck squamous cell carcinoma


Tripti Verma1, Swati Kumari1, Sridhar Mishra1, Madhup Rastogi2, Vandana Tiwari1, Gaurav R Agarwal3, Nidhi Anand1, Nuzhat Husain1 
1 Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Lucknow, Uttar Pradesh, India
2 Department of Radiotherapy, Dr. Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Lucknow, Uttar Pradesh, India
3 Department of Radiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Lucknow, Uttar Pradesh, India

Correspondence Address:
Nuzhat Husain
Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow - 226 010, Uttar Pradesh
India

Context: Liquid biopsy has moved from bench to bedside as a non-invasive biomarker for early diagnosis and monitoring treatment response. Objective: This study investigated the role of circulating free DNA (cfDNA) as a diagnostic marker in locally advanced head and neck squamous cell carcinoma (HNSCC) and in monitoring response to chemoradiation therapy. Materials and Methods: Serum was collected from treatment naïve, histopathologically diagnosed tumors in 24 HNSCC cases and 16 normal controls. CfDNA levels were quantified using β globin gene amplification. Results: The cfDNA level was significantly elevated in HNSCC (992.67 ± 657.43 ng/mL) as compared to healthy controls (60.65 ± 30.42 ng/mL, P = <0.001). The levels of cfDNA did not significantly correlate with TNM stage, lymph node involvement and grade. In responders, percentage decrease in cfDNA levels was 9.57% and 29.66%, whereas in nonresponders percentage increase was 13.28% and 24.52% at the end of three months of follow-up. Conclusion: Our study adds to the evidence that cfDNA levels are significantly higher in HNSCC cases and provides some evidence that levels increase with tumor progression. CfDNA may be a promising prospective non-invasive marker to predict response in patients undergoing chemo-radiotherapy.


How to cite this article:
Verma T, Kumari S, Mishra S, Rastogi M, Tiwari V, Agarwal GR, Anand N, Husain N. Circulating free DNA as a marker of response to chemoradiation in locally advanced head and neck squamous cell carcinoma.Indian J Pathol Microbiol 2020;63:521-526


How to cite this URL:
Verma T, Kumari S, Mishra S, Rastogi M, Tiwari V, Agarwal GR, Anand N, Husain N. Circulating free DNA as a marker of response to chemoradiation in locally advanced head and neck squamous cell carcinoma. Indian J Pathol Microbiol [serial online] 2020 [cited 2020 Nov 30 ];63:521-526
Available from: https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2020;volume=63;issue=4;spage=521;epage=526;aulast=Verma;type=0