Figure 1: (a) Western blot analysis of breast cancer tissue sample: Cell lysate from Membrane fractions from Graves' thyroid (lane 1), breast carcinoma tissue (lane 2,3), and from breast cancer MCF-7 cells. Two bands corresponding to 77 kda and 55 kda hNIS were detected. (b) Intracellular localization of hNIS: Breast cancer membrane fraction (lane 1, 3) and cytosolic fraction (lane 2,4) prepared from tissue samples Presence of two 77 kda and 55 kda forms were detected. (c) Graves' disease tissue thyroid showed membrane and cytoplasmic positivity of moderate to strong intensity, (d) peritumoral and normal breast tissue showed predominantly NIS negative epithelium, (e) Fibrocystic disease of breast showed basolateral, membrane positivity of mild to moderate intensity. (c-e, ×20)