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  Indian J Med Microbiol
 

Figure 1: Mutation detection by MassARRAY and Sanger sequencing. Primer extension products were detected by mass spectrometry, including (a) CTNNB1 S45F, (b) KRAS G12S, (c) PIK3CA E545K, and (d) TP53 R273C. The positions of primer extension products with wildtype and mutant alleles are indicated. (e) Sanger sequence detection of the TP53 R273 mutation depicted in (d)

Figure 1: Mutation detection by MassARRAY and Sanger sequencing. Primer extension products were detected by mass spectrometry, including (a) CTNNB1 S45F, (b) KRAS G12S, (c) PIK3CA E545K, and (d) TP53 R273C. The positions of primer extension products with wildtype and mutant alleles are indicated. (e) Sanger sequence detection of the TP53 R273 mutation depicted in (d)