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  Indian J Med Microbiol
 

Figure 2: (a and b) Fluorescent in situ hybridization (FISH) using XY probe on bone marrow and peripheral blood (PB). (a) Metaphase and interphase FISH on bone marrow aspirate cultures from case 1 using CEP X Spectrum orange/Y Spectrum green probe (Vysis, Abbott ) shows loss of chromosome Y (LOY) in the metaphase and normal pattern in the interphase cells (×1000 oil immersion). (b) Interphase FISH on PB from case 1 using CEP X Spectrum orange/Y Spectrum green probe (Vysis, Abbott ) shows normal signal pattern suggesting no LOY (×1000 oil immersion)

Figure 2: (a and b) Fluorescent <i>in situ</i> hybridization (FISH) using XY probe on bone marrow and peripheral blood (PB). (a) Metaphase and interphase FISH on bone marrow aspirate cultures from case 1  using CEP X Spectrum orange/Y Spectrum green probe (Vysis, Abbott ) shows loss of chromosome Y (LOY) in the metaphase and normal pattern in the interphase cells (×1000 oil immersion). (b) Interphase FISH on PB from case 1 using CEP X Spectrum orange/Y Spectrum green probe (Vysis, Abbott ) shows normal signal pattern suggesting no LOY (×1000 oil immersion)