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  Indian J Med Microbiol
 

Figure 1: Agarose gel analysis for the detection of JAK2V617F mutation in genomic DNA by amplification refractory mutation screening-polymerase chain reaction assay. Lane 1, 4, 5, 6 and 8 show a normal genotype, Lane 2 and 3 show a single mutant band (279 bp) and were scored as homozygous for the mutation; Lane 7 showed mutant band of similar intensity to the normal band and was therefore scored as heterozygous; the Lane 9 is negative control and M is 100 bp ladder. Arrows indicate the product of amplification

Figure 1: Agarose gel analysis for the detection of JAK2V617F mutation in genomic DNA by amplification refractory mutation screening-polymerase chain reaction assay. Lane 1, 4, 5, 6 and 8 show a normal genotype, Lane 2 and 3 show a single mutant band (279 bp) and were scored as homozygous for the mutation; Lane 7 showed mutant band of similar intensity to the normal band and was therefore scored as heterozygous; the Lane 9 is negative control and M is 100 bp ladder. Arrows indicate the product of amplification