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  Indian J Med Microbiol
 

Figure 2: (a) Multiplex immunohistochemistry CD4+ CD8 cocktail: Atypical T-lymphoid cells show strong red positivity for CD 8 and are CD4 negative (×100). (b) Immunohistochemistry - CD 30 is negative (×400). (c) Polyacrylamide gel for T-cell receptor gamma gene rearrangement. Lane 1: 100 bp ladder; Lane 2: Positive or monoclonal control (167 bp); Lane 3: Specimen control ladder (presence of all the products indicate the integrity of DNA). Lane 4: T-cell receptor gamma MMx-A (shows monoclonal band); Lane 5: T-cell receptor gamma MMx-B; Lane 6: Negative or polyclonal control

Figure 2: (a) Multiplex immunohistochemistry CD4+ CD8 cocktail: Atypical T-lymphoid cells show strong red positivity for CD 8 and are CD4 negative (×100). (b) Immunohistochemistry - CD 30 is negative (×400). (c) Polyacrylamide gel for T-cell receptor gamma gene rearrangement. Lane 1: 100 bp ladder; Lane 2: Positive or monoclonal control (167 bp); Lane 3: Specimen control ladder (presence of all the products indicate the integrity of DNA). Lane 4: T-cell receptor gamma MMx-A (shows monoclonal band); Lane 5: T-cell receptor gamma MMx-B; Lane 6: Negative or polyclonal control